2013 Fiscal Year Final Research Report
Establishment of early diagnosis system of pancreatobiliary carcinomas by the application of mucin expression profiles
| Project/Area Number |
23390085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kagoshima University |
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Principal Investigator |
YONEZAWA Suguru 鹿児島大学, 医歯(薬)学総合研究科, 教授 (10175002)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHI Michiyo 鹿児島大学, 医歯学総合研究科, 准教授 (60315405)
YOKOYAMA Seiya 鹿児島大学, 医歯学総合研究科, 助教 (20569941)
SHINCHI Hiroyuki 鹿児島大学, 医歯学総合研究科, 教授 (60284874)
NARIMATSU Hisashi 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, センター長 (40129581)
KAMEYAMA Akihiko 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, チーム長 (80415661)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 膵癌 / IPMN / mucin / DNA methylation / diagnosis / angiogenesis |
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| Research Abstract |
It is very difficult to perform an early detection of pancreatic ductal adenocarcinomas (PDACs) or an accurate differential diagnosis for subtypes of intraductal papillary mucinous neoplasms (IPMNs) with different outcome. Expression profiles of MUC1, MUC2 and MUC4 are different between PDACs and IPMNs. We developed a methylation-specific electrophoresis (MSE) method to analyze the DNA methylation status.
By using the MSE, we evaluated pancreatic juices from 45 patients. The predictive diagnosis by MSE showed sensitivity and specificity of 80% and 87% for PDAC; 88% and 100% for intestinal-type IPMN; and 77% and 88% for gastric-type IPMN; respectively. The MSE analysis of pancreatic juices may give us useful informations for diagnosis of human pancreatic neoplasms. Another study for the first time demonstrated a pivotal role of MUC1 in controlling the hypoxia-driven angiogenesis through the regulation of multiple proangiogenic factors in PDAC cells.
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Research Products
(25 results)
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[Journal Article] Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice2014
Author(s)
Yokoyama S, Kitamoto S, Higashi M, Goto Y, Hara T, Ikebe D, Yamaguchi T, Arisaka Y, Niihara, Nishimata H, Tanaka S, Takaori K, Batra S K, Yonezawa S
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Journal Title
PLoS ONE
Volume: 9(4)
Pages: e93760
DOI
Peer Reviewed
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[Journal Article] Pathobiological implications of mucin (MUC) expression in the outcome of small bowel cancer2014
Author(s)
Shibahara H, Higashi M, Koriyama C, Yokoyama S, Kitazono I, Yasuhiro K, Narita M, Kuze S, Takanori K, Mita S, Arai T, Kato T, Yuasa N, Yamaguchi R, Kubota H, Suzuki H, Baba S, Rousseau K, Batra S K, Yonezawa S
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Journal Title
PLoS ONE
Volume: 9(4)
Pages: e86111
DOI
Peer Reviewed
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[Journal Article] Expression of MUC4 mucin is observed mainly in the intestinal-type of intraductal papillary mucinous neoplasm of the pancreas2013
Author(s)
Kitazono I, Higashi M, Kitamoto S, Yokoyama S, Horinouchi M, Osako M, Shimizu T, Tabata M, Batra S K, Goto M, Yonezawa S
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Journal Title
Pancreas
Volume: 42(7)
Pages: 1120-1128
DOI
Peer Reviewed
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[Journal Article] MUC4 and MUC1 expression in adenocarcinoma of the stomach correlates with vessel invasion and lymph node metastasis : an immunohistochemical study of early gastric cancer2012
Author(s)
Tamura Y, Higashi M, Kitamoto S, Yokoyama S, Osako M, Horinouchi M, Shimizu T, Tabata M, Batra SK, Goto M, Yonezawa S
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Journal Title
PLoS ONE
Volume: 7(11)
Pages: e49251
DOI
Peer Reviewed
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[Journal Article] A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain : use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach2012
Author(s)
Yonezawa S, Kitajima S, Higashi M, Osako M, Horinouchi M, Yokoyama S, Kitamoto S, Yamada N, Tamura Y, Shimizu T, Tabata M, Goto M
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Journal Title
Gastric Cancer
Volume: 15(4)
Pages: 370-381
DOI
Peer Reviewed
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[Journal Article] Prognostic relevance of morphological types of intraductal papillary mucinous neoplasms of the pancreas2011
Author(s)
Furukawa T, Hatori T, Fujita I, Yamamoto M, Kobayashi M, Ohike N, Morohoshi T, Egawa S, Unno M, Takao S, Osako M, Yonezawa S, Mino-Kenudson M, Lauwers GY, Yamaguchi H, Ban S, Shimizu M
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Journal Title
Gut
Volume: 60(4)
Pages: 509-516
DOI
Peer Reviewed
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[Book] 病理診断に役立つ分子生物学(病理と臨床臨時増刊号 Vol.29) (分担執筆), 第 2 部 病理診断医になじみのある疾患関連分子, MUC 診断編. (金井弥栄, 石川俊平, 池田栄二;編集)2011
Author(s)
米澤 傑, 山田宗茂, 横山勢也, 北本 祥, 田畑和宏, 東美智代
Total Pages
545(334-340)
Publisher
株式会社文光堂, 東京
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