Mechanisms of persistent Helicobacter pylori infection

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390102
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: The University of Tokyo
• Principal Investigator: MIMURO Hitomi 東京大学, 医科学研究所, 准教授 (80396887)
• Co-Investigator(Kenkyū-buntansha): OGAWA Michinaga 国立感染症研究所, 細菌第一部, 室長 (80361624) ; MARUYAMA Fumito 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (30423122)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 細菌 / 感染 / 微生物 / ヘリコバクターピロリ

Research Abstract

The aim of this study was to obtain a comprehensive understanding of the mechanisms underlying Helicobacter pylori (Hp)-induced long-term colonization in stomach. We focused on three aspects of the interplay between Hp and the host: the expression dynamics of Hp, the reaction dynamics at Peyer's patches, and host responses. As a result, we identified candidates of bacterial factors involved in bacterial association with Peyer's patches. We also found that epigenetic silencing of miRNA-X plays a key role in gastric disease progression in response to persistent Hp infection.

Research Products

• [Journal Article] The Immune Receptor NOD1 and Kinase RIP2 Interact with Bacterial Peptidoglycan on Early Endosomes to Promote Autophagy and Inflammatory Signaling (2014)
  • Author(s): Irving AT, Mimuro H, Kufer TA, Lo C, Wheeler R, Turner LJ, Thomas BJ, Malosse C, Gantier MP, Casillas LN, Votta BJ, Bertin J, Boneca IG, Sasakawa C, Philpott DJ, Ferrero RL, Kaparakis-Liaskos M
  • Journal Title: Cell Host Microbe Volume: 15 Pages: 623-635
  • DOI: 10.1016/j.chom.2014.04.001
  • Peer Reviewed
• [Journal Article] Shigella type III secretion protein MxiI is recognized by Naip2 to induce Nlrc4 inflammasome activation independently of Pkcδ (2014)
  • Author(s): Suzuki S, Franchi L, He Y, Muñoz-Planillo R, Mimuro H, Suzuki T, Sasakawa C, Núñez G
  • Journal Title: PL oS Pathog Volume: 10 Pages: e1003926
  • DOI: 10.1371/journal.ppat.1003926
  • Peer Reviewed
• [Journal Article] The Shigella OspC3 effector inhibits caspase-4, antagonizes inflammatory cell death, and promotes epithelial infection (2013)
  • Author(s): Kobayashi T, Ogawa M, Sanada T, Mimuro H H, Kim M, Ashida H, Akakura R, Yoshida M, Kawalec M, Reichhart JM, Mizushima T, Sasakawa C
  • Journal Title: Cell Host Microbe Volume: 13 Pages: 570-583
  • DOI: 10.1016/j.chom.2013.04.012
  • Peer Reviewed
• [Journal Article] A bacterial effector targets the TRAF6-NFκB pathway to modulate the acute inflammatory response to bacterial invasion of epithelial cells (2012)
  • Author(s): Sanada T, Kim M, Mimuro H, Ashida H, Ogawa M, Mizushima T, Sasakawa C
  • Journal Title: Virulence Volume: 3 Pages: 518-521
  • DOI: 10.4161/viru.21451
  • Peer Reviewed
• [Journal Article] Uptake of Shigella -containing pseudopodia by neighboring epithelial cells at tricellular junctions via non-canonical clathrin-dependent trafficking pathway (2012)
  • Author(s): Fukumatsu M, Ogawa M, Kim M, Mimuro H, Sasakawa C
  • Journal Title: Virulence Volume: 3 Pages: 515-518
  • DOI: 10.4161/viru.21740
  • Peer Reviewed
• [Journal Article] Shigella targets epithelial tricellular junctions and uses a noncanonical clathrin-dependent endocytic pathway to spread between cells (2012)
  • Author(s): Fukumatsu M, Ogawa M, Arakawa S, Suzuki M, Nakayama K, Shimizu S, Kim M, Mimuro H H, Sasakawa C
  • Journal Title: Cell Host Microbe Volume: 11 Pages: 325-336
  • DOI: 10.1016/j.chom
  • Peer Reviewed
• [Journal Article] The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response (2012)
  • Author(s): Sanada T, Kim M, Mimuro H, Suzuki M, Ogawa M, Oyama A, Ashida H, Kobayashi T, Koyama T, Nagai S, Shibata Y, Gohda J, Inoue J, Mizushima T, Sasakawa C
  • Journal Title: Nature Volume: 483 Pages: 623-626
  • DOI: 10.1038/nature10894
  • Peer Reviewed
• [Journal Article] Bacteria and host interactions in the gut epithelial barrier (2011)
  • Author(s): Ashida H, Ogawa M, Kim M, Mimuro H, Sasakawa C
  • Journal Title: Nat Chem Biol Volume: 8 Pages: 36-45
  • DOI: 10.1038/nchembio.741
  • Peer Reviewed
• [Journal Article] Cell death and infection : a double-edged sword for host and pathogen survival (2011)
  • Author(s): Ashida H, Mimuro H, Ogawa M, Kobayashi T, Sanada T, Kim M, Sasakawa C
  • Journal Title: J Cell Biol Volume: 195 Pages: 931-942
  • DOI: 10.1083/jcb.201108081
  • Peer Reviewed
• [Journal Article] Shigella are versatile mucosal pathogens that circumvent the host innate immune system (2011)
  • Author(s): Ashida H, Ogawa M, Mimuro H, Kobayashi T, Sanada T, Sasakawa C
  • Journal Title: Curr Opin Immunol Volume: 23 Pages: 448-455
  • DOI: 10.1016/j.coi.2011.06.001
  • Peer Reviewed
• [Journal Article] Attenuated CagA oncoprotein in Helicobacter pylori from Amerindians in Peruvian Amazon (2011)
  • Author(s): Suzuki M, Kiga K, Kersulyte D, Cok J, Hooper CC, Mimuro H, Sanada T, Suzuki S, Oyama M, Kozuka-Hata H, Kamiya S, Zou QM, Gilman RH, Berg DE, Sasakawa C
  • Journal Title: J Biol Chem Volume: 286 Pages: 29964-19972
  • DOI: 10.1074/jbc.M111.263715
  • Peer Reviewed
• [Journal Article] Manipulation of autophagy by bacteria for their own benefit (2011)
  • Author(s): Ogawa M, Mimuro H, Yoshikawa Y, Ashida H, Sasakawa C
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 459-471
  • DOI: 10.1111/j.1348-0421.2011.00343.x
  • Peer Reviewed
• [Journal Article] BabA-mediated adherence is a potentiator of the Helicobacter pylori type IV secretion system activity (2011)
  • Author(s): Ishijima N, Suzuki M, Ashida H, Ichikawa Y, Kanegae Y, Saito I, Borén T, Haas R, Sasakawa C, Mimuro H
  • Journal Title: J Biol Chem Volume: 286 Pages: 25256-25264
  • DOI: 10.1074/jbc.M111.233601
  • Peer Reviewed
• [Journal Article] A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens (2011)
  • Author(s): Ogawa M, Yoshikawa Y, Kobayashi T, Mimuro H, Fukumatsu M, Kiga K, Piao Z, Ashida H, Yoshida M, Kakuta S, Koyama T, Goto Y, Nagatake T, Nagai S, Kiyono H, Kawalec M, Reichhart JM, Sasakawa C
  • Journal Title: Cell Host Microbe Volume: 9 Pages: 376-389
  • DOI: 10.1016/j.chom.2011.04.010
  • Peer Reviewed
• [Presentation] Helicobacter pylori の感染戦略 (2014)
  • Author(s): 三室 仁美
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-28
• [Presentation] ヘリコバクターピロリ感染による宿主応答制御 (2013)
  • Author(s): 三室 仁美
  • Organizer: 平成 25年度遺伝子病制御研究所国際研究集会「Infection, Immunity, Inflammation, Cancer」
  • Place of Presentation: 札幌
  • Year and Date: 2013-10-25
• [Presentation] ヘリコバクターピロリ感染による宿主応答制御 (2013)
  • Author(s): 三室 仁美
  • Organizer: 第19回日本ヘリコバクター学会学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2013-06-29
• [Presentation] Crosstalk between Helicobacter pylori and Host Cells (2012)
  • Author(s): Hitomi Mimuro
  • Organizer: Crosstalk between Helicobacter pylori and Host Cells
  • Place of Presentation: 仙台
  • Year and Date: 2012-09-21
• [Presentation] ヘリコバクターピロリの感染機構 (2012)
  • Author(s): 三室 仁美
  • Organizer: 第65回日本細菌学会九州支部総会
  • Place of Presentation: 沖縄
  • Year and Date: 2012-08-25
• [Presentation] Control of host cell responses by Helicobacter pylori infection (2012)
  • Author(s): Hitomi Mimuro, Kotaro Kiga, and Chihiro Sasakawa
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-28
• [Presentation] Control of host cell responses by Helicobacter pylori infection (2011)
  • Author(s): Hitomi Mimuro, Kotaro Kiga, and Chihiro Sasakawa
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-08
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/Mimuro_Lab/index.html