2013 Fiscal Year Final Research Report
Basic analysis of pathogenesis of whooping cough
| Project/Area Number |
23390104
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMITANI Shigeki 大阪大学, 微生物病研究所, 助教 (60379089)
ABE Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | 百日咳 / 気管支敗血症菌 / 咳嗽 |
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| Research Abstract |
To understand the mechanism of paroxysmal cough observed in whooping cough, Bordetella pertussis infection, we established an animal model that reproduce the paroxysmal cough by using B. bronchiseptica, which is closely related to B. pertussis. This model animal showed coughing after intranasal infection by 500 cfu of B. bronchiseptica. In the course of analyzing the B. bronchiseptica infection, we succeeded in isolating a mutant strain that did not induce coughing in the model animal. A comparison of the genomes of the mutant strain and the parental wild type strain revealed a single-base insertion in a gene designated crg1. A strain deficient in crg1 was found not to cause the paroxysmal cough in the animals, showing that crg1 might be involved in the paroxysmal cough. Further work is in progress to characterize the crg1 gene.
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[Journal Article] Substrate specificity of Pasteurella multocida toxin for α subunits of heterotrimeric G proteins2013
Author(s)
Orth JHC, Fester I, Siegert P, Weise M, Lanner U, Kamitani S, Tachibana T, Wilson BA, Schlosser A, Horiguchi Y, Aktories K
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Journal Title
FASEB J
Volume: 27
Pages: 832-842
DOI
Peer Reviewed
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[Journal Article] Crystal Structure of Clostridium perfringens Enterotoxin Displays Features of {beta}-Pore-forming Toxins2011
Author(s)
Kitadokoro K, Nishimura K, Kamitani S, Fukui-Miyazaki A, Toshima H, Abe H, Kamata Y, Sugita-Konishi Y, Yamamoto S, Karatani H, Horiguchi Y
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Journal Title
J Biol Chem
Volume: 286
Pages: 19549-19555
DOI
Peer Reviewed
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