2014 Fiscal Year Final Research Report
Molecular machinery for recognition of viral ribonucleoprotein complex (RNP) by host innate immune receptors
| Project/Area Number |
23390108
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Keywords | ウイルス / 自然免疫 / RNA |
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| Outline of Final Research Achievements |
In this research project, we tried to reveal how viral RNA sensors, RIG-I-like receptors (RLRs), recognize replication-competent viral ribonucleoprotein complexes (RNPs) that are formed in virus-infected cells. We established in vitro reconstitution assay system to detect activation of RIG-I-mediated signaling in response to substrate RNAs, such as 5’-triphosphate-containing synthetic RNAs. As a result of investigations using viral RNPs of influenza A virus (IAV) as substrates, we clearly observed activation of RIG-I-mediated signaling by IAV RNPs in vitro. These results could help us to understand how anti-viral innate immune responses are initiated in virus-infected cells.
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| Free Research Field |
分子生物学
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