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2013 Fiscal Year Final Research Report

Study on pathophysiological roles of brain NO-NCX signal

Research Project

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Project/Area Number 23390139
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied pharmacology
Research InstitutionOsaka University

Principal Investigator

MATSUDA Toshio  大阪大学, 薬学研究科(研究院), 教授 (00107103)

Co-Investigator(Kenkyū-buntansha) 吾郷 由希夫  大阪大学, 薬学研究科, 大阪大学 (50403027)
TAKUMA Kazuhiro  大阪大学, 薬学研究科, 准教授 (90289025)
Research Collaborator HASHIMOTO Hitoshi  大阪大学, 薬学研究科, 教授 (30240849)
Project Period (FY) 2011-04-01 – 2014-03-31
KeywordsNa+/Ca2+交換系 / 一酸化窒素 / 環境要因
Research Abstract

This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in HEK293 cell expression system. Transfection with a plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA into HEK293 cells increased prominently the protein level of NCX1.5 tagged with C-terminal V5 and (His)6 epitopes (NCX1.5-V5/His). There was no difference in the cytotoxic effects by sodium nitroprusside and S-nitroso-N-acetylpenicillamine between the control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5. Microdialysis study showed that NO did not affect monoamine release in the brain. Furthermore, RT-PCR analysis showed that environmental factors did not affect the expression of NCX.

  • Research Products

    (23 results)

All 2014 2013 2012 2011

All Journal Article (16 results) (of which Peer Reviewed: 15 results) Presentation (7 results)

  • [Journal Article] The effect of high [K+]o on spontaneous Ca2+ waves in freshly isolated interstitial cells of Cajal from the rabbit urethra2014

    • Author(s)
      Drumm BT, Sergeant GP, Hollywood MA, Thornbury KT, Matsuda T, Baba A, Harvey BJ, McHale NG
    • Journal Title

      Physiol Rep

      Volume: 2(1) Pages: e00203

    • Peer Reviewed
  • [Journal Article] Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells2013

    • Author(s)
      Ota Y, Kawanai T, Watanabe R, Nishimura A, Ago Y, Takuma K, Matsuda T
    • Journal Title

      J Pharmacol Sci

      Volume: 121(4) Pages: 351-354

    • Peer Reviewed
  • [Journal Article] Effects of SEA0400 on Arrhythmogenicity in a Langendorff- perfused 1-month myocardial infarc- tion rabbit model2013

    • Author(s)
      Chou CC, Chang PC, Wen MS, Lee HL, Chu Y, Baba A, Matsuda T, Yeh SJ, Wu D
    • Journal Title

      Pacing Clin Electro- physiol

      Volume: 36(5) Pages: 596-606

    • Peer Reviewed
  • [Journal Article] Pretreatment of donor islets with the Na+/Ca2+ exchanger inhibitor improves the efficiency of islet transplantation2013

    • Author(s)
      Mera T, Itoh T, Kita S, Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y
    • Journal Title

      Am J Transplant

      Volume: 13(8) Pages: 2154-2160

    • Peer Reviewed
  • [Journal Article] Stimulation of Na+/H+ exchanger isoform 1 promotes microglial migration2013

    • Author(s)
      Shi Y, Yuan H, Kim D, Chanana V, Baba A, Matsuda T, Cengiz P, Ferrazzano P, Sun D
    • Journal Title

      PLoS One

      Volume: 8(8) Pages: e74201

    • Peer Reviewed
  • [Journal Article] The glial sodium-calcium exchanger : a new target for nitric oxide-mediated cellular toxicity2013

    • Author(s)
      Takuma K, Ago Y, Matsuda T
    • Journal Title

      Curr Protein Pept Sci

      Volume: 14 Pages: 43-50

    • Peer Reviewed
  • [Journal Article] グリア細胞Na+/Ca2+交換系の病態生理学的役割2013

    • Author(s)
      田熊一敞, 吾郷由希夫, 松田敏夫
    • Journal Title

      生体の科学(医学書院)

      Volume: 64(5) Pages: 384-385

  • [Journal Article] Neuronal nitric oxide synthase inhibition attenuates the development of L-DOPA-induced dyskinesia in hemi-Parkinsonian rats2012

    • Author(s)
      Takuma K, Tanaka T, Takahashi T, Hiramatsu N, Ota Y, Ago Y, Matsuda T
    • Journal Title

      Eur J Pharmacol

      Volume: 683(1-3) Pages: 166-173

    • Peer Reviewed
  • [Journal Article] Astrocytes modulate neural network activity by Ca² -dependent uptake of extracellular K2012

    • Author(s)
      Wang F, Smith NA, Xu Q, Fujita T, Baba A, Matsuda T, Takano T, Bekar L, Nedergaard M
    • Journal Title

      Sci Signal

      Volume: 5(218) Pages: 26

    • Peer Reviewed
  • [Journal Article] Revisiting the Ionic Mechanisms of Early Afterdepolarizations in Cardiomyo- cytes : Predominant by Ca Waves or Ca Currents?2012

    • Author(s)
      Zhao Z, Wen H, Fefelova N, Allen C, Baba A, Matsuda T, Xie LH
    • Journal Title

      Am J Physiol Heart Circ Physiol

      Volume: 302(8) Pages: H1636-1644

    • Peer Reviewed
  • [Journal Article] Na delivery and ENaC mediate flow regulation of collecting duct endothelin-1 production2012

    • Author(s)
      Pandit MM, Strait KA, Matsuda T, Kohan DE
    • Journal Title

      Am J Physiol Renal Physiol

      Volume: 302(10) Pages: F1325-1330

    • Peer Reviewed
  • [Journal Article] Inhibition of the sodium-calcium exchanger via SEA0400 altered manganese-induced T(1) changes in isolated perfused rat hearts2012

    • Author(s)
      Chen Y, Payne K, Perara VS, Huang S, Baba A, Matsuda T, Yu X
    • Journal Title

      NMR Biomed

      Volume: 25(11) Pages: 1280-1285

    • Peer Reviewed
  • [Journal Article] New experimental evidence for mechanism of arrhythmogenic membrane potential alternans based on balance of electrogenic INCX/ICa currents2012

    • Author(s)
      Wan X, Cutler M, Song Z, Karma A, Matsuda T, Baba A, Rosenbaum DS
    • Journal Title

      Heart Rhythm

      Volume: 9(10) Pages: 1698-705

    • Peer Reviewed
  • [Journal Article] Preferential involvement of Na+/Ca2+ exchanger type-1 in the brain damage caused by transient focal cerebral ischemia in mice2012

    • Author(s)
      Morimoto N, Kita S, Shimazawa M, Namimatsu H, Tsuruma K, Hayakawa K, Mishima K, Egashira N, Iyoda T, Horie I, Gotoh Y, Iwasaki K, Fujiwara M, Matsuda T, Baba A, Komuro I, Horie K, Takeda J, Iwamoto T, Hara H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 429(3-4) Pages: 186-190

    • Peer Reviewed
  • [Journal Article] SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease2011

    • Author(s)
      Ago Y, Kawasaki T, Nashida T, Ota Y, Cong Y, Kitamoto M, Takahashi T, Takuma K, Matsuda T
    • Journal Title

      Neuropharmacology

      Volume: 61(8) Pages: 1441-1451

    • Peer Reviewed
  • [Journal Article] Ca2+ influx through reverse-mode Na+/Ca2+ exchange is critical for vascular endothelial growth factor (VEGF) mediated - ERK1/2 activation and angiogenic functions of human endothelial cells2011

    • Author(s)
      Andrikopoulos P, Baba A, Matsuda T, Djamgoz MB, Yaqoob MM, Eccles SA
    • Journal Title

      J Biol Chem

      Volume: 286(44) Pages: 37919-37931

    • Peer Reviewed
  • [Presentation] PACAP 遺伝子欠損マウスの異常行動に対する自発運動の作用2013

    • Author(s)
      中川 光, 福山留以, 山口浩史, 新谷紀人, 橋本均, 吾郷由希夫, 田熊一敞, 松田敏夫
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡
    • Year and Date
      20130321-23
  • [Presentation] 幼若期環境強化によるPACAP 遺伝子欠損マウス学習記憶障害の発現抑制2012

    • Author(s)
      中川光, 竹本光佑, 福山留以, 新谷紀人, 橋本均, 吾郷由希夫, 田熊一敞, 松田敏夫
    • Organizer
      生体機能と創薬シンポジウム2012
    • Place of Presentation
      神戸
    • Year and Date
      20120830-31
  • [Presentation] レボドパ誘発ジスキネジア形成過程における神経型一酸化窒素合成酵素の役割2012

    • Author(s)
      田熊一敞, 田中辰典, 高橋剛士, 太田友樹, 平松直樹, 吾郷由希夫, 松田敏夫
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      20120328-31
  • [Presentation] 6-Hydroxydopamine 神経障害ラットにおけるL-DOPA 誘発ジスキネジアの形成に対する神経型一酸化窒素合成酵素阻害薬7-nitroindazole の改善効果2012

    • Author(s)
      太田友樹, 田熊一敞, 高橋剛士, 吾郷由希夫, 松田敏夫
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      京都
    • Year and Date
      20120314-16
  • [Presentation] 発育期環境要因のマウス精神機能発達に対する作用2012

    • Author(s)
      田中辰典, 吾郷由希夫, 井本絵実奈, 北本真理, 田熊一敞, 松田敏夫
    • Organizer
      第62回日本薬学会近畿支部総会・大会
    • Place of Presentation
      神戸
    • Year and Date
      2012-10-20
  • [Presentation] PACAP 欠損マウスの学習記憶障害に対する豊かな環境飼育の影響2012

    • Author(s)
      福山留以, 竹本光佑, 中川 光, 新谷紀人, 橋本均, 吾郷由希夫, 田熊一敞, 松田敏夫
    • Organizer
      第121回日本薬理学会近畿部会
    • Place of Presentation
      徳島
    • Year and Date
      2012-06-29
  • [Presentation] 神経型一酸化窒素合成酵素阻害剤7-nitroindazole は6-hydroxydopamine 神経障害ラットにおいてL-DOPA 誘発ジスキネジアの形成を抑制する2011

    • Author(s)
      太田友樹, 田熊一敞, 高橋剛士, 吾郷由希夫, 松田敏夫
    • Organizer
      第54回日本神経化学会大会
    • Place of Presentation
      石川
    • Year and Date
      20110926-28

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Published: 2015-06-25  

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