Development of method for the systemic separation of fetal membrane-derived MSCs to establish the cell-specific transplantation therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 23390145
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: IKEDA TOMOAKI 独立行政法人国立循環器病研究センター, 病院, 客員部長 (80202894)
• Co-Investigator(Kenkyū-buntansha): YAMAHARA Kenichi 独立行政法人国立循環器病研究センター, 研究所, 室長 (50450888) ; OTANI Kentaro 独立行政法人国立循環器病研究センター, 研究所, 研究員 (50470191) ; INUBUSHI Masayuki 川崎医科大学, 医学部, 准教授 (70399830)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 細胞・組織 / 間葉系幹細胞 / 胎児付属物 / 卵膜 / 細胞シート / 他家移植

Outline of Final Research Achievements

We aimed to develop the method to separate fetal membrane-derived MSCs, and to establish the optimized cell-based therapy for the application in a variety of diseases.
1. Basic research for the clinical application of fetal membrane-derived MSC transplantation;○Analysis of therapeutic effects in refractory disease models after fetal membrane-derived MSC transplantation: rat artificial lung and mouse SLE models.○Establishment of the method for cell separation and mass culture: In National Cardiovascular Research Center Cell Processing Center (CPC), we established a method to generate GMP-grade human fetal membrane-derived MSCs, and this technique was transferred to Hokkaido University Hospital CPC.
2. Pre-clinical trials by fetal membrane-derived MSC transplantation○Toxicity test of fetal membrane-derived MSCs in large animals: Fetal-membrane-derived MSCs were intravenously injected in pig and no hemodynamic change was observed.

Free Research Field

再生医療、産婦人科学