Application of novel clinicopathological biomarkers to cancer treatment

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Shiga University of Medical Science
• Principal Investigator: OKABE Hidetoshi 滋賀医科大学, 医学部, 教授 (70079713)
• Co-Investigator(Kenkyū-buntansha): CHANO Tokuhiro 滋賀医科大学, 医学部, 准教授 (40346028) ; ISONO Takahiro 滋賀医科大学, 医学部, 准教授 (20176259)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 臨床病理 / 分子標的治療 / バイオマーカー / siRNA / 癌

Research Abstract

In salivary gland cancers, RB1CC1 was demonstrated to be a useful prognostic predictor. In head and neck carcinomas, QL1 & WRN DNA helicase were therapeutic targetting molecules, and the siRNA silencing could result specifically cell death in the cancers. p62/SQSTM1 was highly expressed in head and neck carcinomas. p62/SQSTM1 could induce GSH, cause a resistance to oxidative stress, be associated with a resisitance to radiation therapy, and finally predict the worse prognosis. In testicular embryonal carcinomas, SOX2-siRNA caused significant inhibition for the tumor growth in vivo, suggesting that it will be a promising therapeutic target in the future.

Research Products

• [Journal Article] Glucose deprivation induces G2/M transition-arrest and cell death in N-GlcNAc2-modified protein-producing renal carcinoma cells (2014)
  • Author(s): Isono T, Chano T, Kitamura A, Yuasa T
  • Journal Title: PLoS One Volume: 9(5) Pages: e96168
  • Peer Reviewed
• [Journal Article] Association of p62/SQSTM1 excess and oral carcinogenesis (2013)
  • Author(s): Inui T, Chano T, Takikita M, Nishikawa M, Yamamoto G, Okabe H
  • Journal Title: PLoS One Volume: 8 (9) Pages: e74398
  • Peer Reviewed
• [Journal Article] Study of Global Transcriptional Changes of N-GlcNAc2 Proteins-Producing T24 Bladder Carcinoma Cells under Glucose Deprivation (2013)
  • Author(s): Isono T, Chano T, Okabe H, Suzaki M
  • Journal Title: PLoS One Volume: 8(4) Pages: e60397.
  • Peer Reviewed
• [Journal Article] Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application (2012)
  • Author(s): Hama Y, Chano T, Inui T, Matsumoto K, Okabe H
  • Journal Title: PLoS One Volume: 7(3) Pages: e32052
  • Peer Reviewed
• [Journal Article] Therapeutic potential of SOX2 inhibition in embryonal carcinoma (2012)
  • Author(s): Ushida H, Chano T, Minami K, Kita H, Kawakami T, Okabe H, Okada Y, Okamoto K
  • Journal Title: J Urology Volume: 187(5) Pages: 1876-1881
  • Peer Reviewed
• [Journal Article] Prognostic significance of RB1-inducible coiled-coil 1 in salivary gland cancers (2012)
  • Author(s): Tameno H, Chano T, Ikebuchi K, Ochi Y, Arai A, Kishimoto M, Shimada T, Hisa Y, Okabe H
  • Journal Title: Head Neck Volume: 34(5) Pages: 674-680
  • Peer Reviewed
• [Journal Article] Suppression of viral replication by drs tumor suppressor via mTOR dependent pathway (2012)
  • Author(s): Tambe Y, Okuyama N, Nakagawa T, Muramoto A, Hasebe M, Chano T, Inoue H
  • Journal Title: Cancer Lett Volume: 314(1) Pages: 82-91
  • Peer Reviewed
• [Journal Article] Hyperbranched Polyglycerol-Grafted Superparamagnetic Iron Oxide Nanoparticles : Synthesis, Characterization, Functionalization, Size Separation, Magnetic Properties, and Biological Applications (2012)
  • Author(s): Zhao L, Chano T, Morikawa S, Saito Y, Shiino A, Shimizu S, Maeda T, Irie T, Aonuma S, Okabe H, Kimura T, Inubushi T, Komatsu N
  • Journal Title: Adv Funct Mater Volume: 22(24) Pages: 5107-5117
  • Peer Reviewed
• [Journal Article] A global transcriptome analysis of a dog model of congestive heart failure with the human genome as a reference (2012)
  • Author(s): Isono T, Matsumoto T, Wada A, Suzaki M, Chano T
  • Journal Title: J Card Fail Volume: 18(1) Pages: 872-878
  • Peer Reviewed
• [Journal Article] 診療における新規マーカーの探索と適用 (2012)
  • Author(s): 茶野徳宏
  • Journal Title: 臨床病理 Volume: 60(2) Pages: 167-173
• [Journal Article] RECQL1 and WRN proteins are potential therapeutic targets in head and neck squamous cell carcinoma (2011)
  • Author(s): Arai A, Chano T, Futami K, Furuichi Y, Ikebuchi K, Inui T, Tameno H, Ochi Y, Shimada T, Hisa Y, Okabe H
  • Journal Title: Cancer Res Volume: 71 (13) Pages: 4598-4607
  • Peer Reviewed
• [Journal Article] RB1CC1 activates the p16 promoter through the interaction with hSNF5 (2011)
  • Author(s): Ochi Y, Chano T, Ikebuchi K, Inoue H, Isono T, Arai A, Tameno H, Shimada T, Hisa Y, Okabe H
  • Journal Title: Oncol Rep Volume: 26(4) Pages: 805-812
  • Peer Reviewed
• [Journal Article] p53 inhibits autophagy by interacting with the human ortholog of yeast Atg17, RB1CC1/FIP200 (2011)
  • Author(s): Morselli E, Shen S, Ruckenstuhl C, Bauer MA, Mariño G, Galluzzi L, Criollo A, Michaud M, Maiuri MC, Chano T, Madeo F, Kroemer G
  • Journal Title: Cell Cycle Volume: 10(16) Pages: 2763-2769
  • Peer Reviewed
• [Journal Article] RB1CC1 protein positively regulates transforming growth factor-beta signaling through the modulation of Arkadia E3 ubiquitin ligase activity (2011)
  • Author(s): Koinuma D, Shinozaki M, Nagano Y, Ikushima H, Horiguchi K, Goto K, Chano T, Saitoh M, Imamura T, Miyazono K, Miyazawa K
  • Journal Title: J Biol Chem Volume: 286(37) Pages: 32502-32512
  • Peer Reviewed
• [Journal Article] RB1CC1 protein suppresses type II collagen synthesis in chondrocytes and causes dwarfism (2011)
  • Author(s): Nishimura I, Chano T, Kita H, Matsusue Y, Okabe H
  • Journal Title: J Biol Chem Volume: 286(51) Pages: 43925-43932
  • Peer Reviewed
• [Journal Article] Methylation profile of DNA repetitive elements in human testicular germ cell tumor (2011)
  • Author(s): Ushida H, Kawakami T, Minami K, Chano T, Okabe H, Okada Y, Okamoto K
  • Journal Title: Mol Carcinogen Volume: 51(9) Pages: 711-722
  • Peer Reviewed
• [Journal Article] Balance between S6K-S6 and 4E-BP1 depends on ERK activity in developing neurons (2011)
  • Author(s): Nishimura I, Mori K, Matsusue Y, Okabe H, Chano T
  • Journal Title: Curr Signal Transd T Volume: 6(1) Pages: 82-87
  • Peer Reviewed
• [Remarks] 2011年6月7日中日新聞(朝刊), 京都新聞(朝刊)(新聞報道)
• [Remarks] 2011年6月10日産経新聞(朝刊)(新聞報道)
• [Remarks] 2011年7月7日朝日新聞(朝刊)(新聞報道)
• [Remarks] 2013年8月24日中日新聞(朝刊), 産經新聞(夕刊)(新聞報道)
• [Remarks] 2013年8月27日朝日新聞(朝刊)(新聞報道)
• [Remarks] 2013年8月30日京都新聞(朝刊)(新聞報道)
• [Remarks] 2013年8月30日毎日新聞(朝刊)(新聞報道)
• [Patent(Industrial Property Rights)] 頭頸部癌及び食道癌用抗癌剤及び増強剤 (2011)
  • Inventor(s): 茶野徳宏, 島田剛敏, 二見和伸, 古市泰宏
  • Industrial Property Rights Holder: 茶野徳宏, 島田剛敏, 二見和伸, 古市泰宏
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2011-089089
  • Filing Date: 2011-04-13