2013 Fiscal Year Final Research Report
Study on the development of molecules for depressing neuropathic pain based on tertiary structure of human P2X4 receptor
| Project/Area Number |
23390155
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Kyushu University |
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Principal Investigator |
UEDA Tadashi 九州大学, 薬学研究科(研究院), 教授 (90184928)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Yoshito 九州大学, 大学院・薬学研究院, 准教授 (60315091)
TANAKA Hiroyuki 九州大学, 大学院・薬学研究院, 准教授 (30253470)
SHIROISH Mitsunori 九州大学, 大学院・薬学研究院, 助教 (00380527)
SAITO Hidetoshi 九州大学, 大学院・薬学研究院, 助教 (90444794)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Kazuhide 九州大学, 大学院・薬学研究院, 教授 (80124379)
TSUDA Makoto 九州大学, 大学院・薬学研究院, 准教授 (40373394)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 抗体 / 神経障害性疼痛 |
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| Research Abstract |
We planed to develop molecules for depressing neuropathic pain involved in P2X4 receptor. Since we prepared a monoclonal antibody bound to rat P2X4 extracellular region, we planed to prepare monoclonal antibodies bound to human P2X4 extracellular regions using similar methods. Although a couple of types of monoclonal antibodies bound to human P2X4 extracellular regions were prepared, they did not have a potential to depress neuropathic pain. We newly developed the method to evaluate the interaction of a monoclonal antibody with a functionally trimeric P2X4 receptor. Using the method, it was shown that prepared monoclonal antibodies prepared in this study did not bind to a functionally trimeric P2X4 receptor, which may be the reason why monoclonal antibodies bound to human P2X4 extracellular regions did not have a potential to depress neuropathic pain.
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