2013 Fiscal Year Final Research Report
Investigation of role for MafB in the pathogenesis of chronic obstructive pulmonary disease using MafB gene targeted mice
| Project/Area Number |
23390220
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
SHIBATA Yoko 山形大学, 医学部, 講師 (60333978)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUROTANI Reiko 山形大学, 大学院・理工学研究科, 助教 (00453043)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
ABE Shuichi 山形大学, 医学部, 助教 (40400543)
INOUE Sumito 山形大学, 医学部, 助教 (70466621)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | COPD / MafB / マクロファージ / 喫煙 / 転写因子 / マトリックスメタロプロテアーゼ / エラスターゼ肺気腫 |
|---|
| Research Abstract |
Role of MafB in the pathogenesis of chronic obstructive pulmonary disease (COPD) was investigated using macrophage scavenger receptor promoter dominant negative-MafB transgenic (MSR-DN-MafB TG) mice. Mean linear intercept after intratracheal administration of elastase was significantly reduced in TG mice compared to the wild type mice (WT). In TG mice, number of alveolar macrophages (AMs) with projected pseudopods was significantly reduced. In addition, number of F4/80 intermediately positive and CD11b weakly or intermediately positive AMs, considerable cell subsets of matured AMs, decreased in BAL cells of DN-MafB Tg mice. Furthermore, the expression of MMP-9 and -12 was suppressed significantly in BAL cells of DN-MafB Tg mice. Because MMPs exacerbate emphysema, MafB may be involved in pulmonary emphysema pathogenesis through altering the maturation of macrophages and the expression of MMPs.
|
