2014 Fiscal Year Final Research Report
Molecular targeted drug discovery for cancer cell radiosensitization using RNA technology
Project/Area Number |
23390301
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Radiation science
|
Research Institution | Tottori University |
Principal Investigator |
KURIMASA Akihiro 鳥取大学, 医学(系)研究科(研究院), 准教授 (80343276)
|
Co-Investigator(Kenkyū-buntansha) |
OKAYASU Ryuichi 放射線医学総合研究所, 放射線防護センターリスク低減化研究プログラム, 主任研究員 (50356135)
|
Project Period (FY) |
2011-04-01 – 2015-03-31
|
Keywords | がん / 放射線 / 放射線生物影響 / RNA工学 / 画像工学 / DNA修復 / 分子標的創薬 |
Outline of Final Research Achievements |
RNA aptamers are artificial synthetic ribonucleic acids that form a higher-order structure and bind phosphorylated sites of specific protein. We will develop novel cancer therapy by utilizing RNA aptamers for controlling radio-sensitivities of malignant cells during radiation therapy for cancer. We tried making RNA aptamers against phosphorylated sites of DNA-PKcs by SELEX, and produced specific molecules to DNA-PK. Some of the RNA enhanced sensitivity of radiation for U2OS cells. In this experiment, we also try to construct an expression system of RNA aptamers using lenti-virus RNA expression vector, and try to find a good candidate molecule for therapy. Furthermore, for developing better radiation therapy, we combine RNA technology and live cell-imaging technology for evaluating DNA damage and cell cycle progression.
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Free Research Field |
放射線腫瘍学
|