Translational research for lung regenerative treatment by cell transplantation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390312
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Chiba University
• Principal Investigator: YOSHINO Ichiro 千葉大学, 医学(系)研究科(研究院), 教授 (40281547)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Shigetoshi 千葉大学, 大学院・医学研究院, 准教授 (90334200) ; MIZOBUCHI Teruaki 千葉大学, 医学部付属病院, 講師 (50569861) ; SAKAIRI Yuichi 千葉大学, 医学部付属病院, 医員 (30551949)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 肺再生 / 細胞移植

Research Abstract

Type II pneumocytes prepared from male F344 rats were effectively integrated in the alveolar tissues of pneumonectomized rats (female), alveolar density of which were recognized to be enhanced (published).As like the above experiments using rats, compensatory lung growth is to be investigated in mice (B6). And also investigated is a relationship between histologic morphology and radiologic density obtained from computed tomography (on going).Human type II pneumocytes were prepared from clinically resected lung, and examined for capability to differentiate to type I pneumocytes and to be integrated in the lung tissue (submitted for publication).To prepare the future lung regeneration medicine, a novel method for estimated quantity of pulmonary tissue was developed (published).Human and murine iPS cells are to be subjected to culture for development of type II pneumocytes (on going).

Research Products

• [Journal Article] Spirometric and radiological evaluation of the remnant lung long after major pulmonary resection : can compensatory phenomena be recognized in clinical cases? (2013)
  • Author(s): Mizobuchi T, Wada H, Sakairi Y, Suzuki H, Nakajima T, Tagawa T, Iwata T, Motoori K, Yoshida S, Yoshino I
  • Journal Title: Surgery Today Surg Today
  • Peer Reviewed
• [Journal Article] Increase of bone-morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats (2013)
  • Author(s): Ohba T, Wada H, Yoshino I, Yoshida S, Tagawa T, Shoji F, Yamazaki K, Maehara Y
  • Journal Title: Surgery Today Volume: 44 Pages: 324-331
  • Peer Reviewed
• [Journal Article] Radiologic evaluation for volume and weight of remnant lung in living lung donors (2013)
  • Author(s): Mizobuchi T, Chen F, Yoshino I, Iwata T, Yoshida S, Bando T, Date H
  • Journal Title: J Thorac Cardiovasc Surg Volume: 146 Pages: 1253-1258
  • Peer Reviewed
• [Journal Article] Transplantation of alveolar type II cells stimulates lung regeneration during compensatory lung growth in adult rats (2012)
  • Author(s): Wada H, Y oshida S, Suzuki H, Sakairi Y, Mizobuchi T, Komura D, Sato Y, Y okoi S, Y oshino I
  • Journal Title: J Thorac Cardiovasc Surg Volume: 143(3) Pages: 711-719
  • Peer Reviewed
• [Presentation] ヒト手術肺からの肺再生療法を目的としたcell sour ceの分離および解析 (2011)
  • Author(s): 坂入祐一, 和田啓伸, 鈴木秀海, 吉田成利, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康光, 星野英久, 岡本龍郎, 本橋新一郎, 吉野一郎
  • Organizer: 第64回日本胸部外科学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20111009-11
• [Presentation] II型肺胞上皮細胞を用いた肺再生療法の開発代償性肺成長モデルによる基礎実験 (2011)
  • Author(s): 和田啓伸, 吉田成利, 鈴木秀海, 坂入祐一, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康光, 星野英久, 岡本龍郎, 本橋新一郎, 河村大輔, 横井左奈, 吉野一郎
  • Organizer: 第47回日本移植学会
  • Place of Presentation: 仙台
  • Year and Date: 20110914-16
• [Presentation] Compensatory Lung Growth Triggered by Major Lung Resection Determined by a Novel Method : Estimated Quantity of Pulmonary Tissue (EQPT) (2011)
  • Author(s): Mizobuchi T, Inage T, Morimoto J, Sakairi Y, Ishibashi F, Chiyo M, Kometani T, Iwata T, Moriya Y, Hoshino H, Yoshida S, Yoshino I
  • Organizer: The annual meeting of American Thoracic Society
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20110513-18
• [Presentation] 全摘術後残存肺における肺再生にはII型肺胞上皮細胞の補充が必要である
  • Author(s): 和田啓伸, 吉田成利, 鈴木秀海, 坂入祐一, 石橋史博, 田村創, 岩田剛和, 溝渕輝明, 守屋康充, 星野英久, 岡本龍郎, 本橋新一郎, 河村大輔, 横井左奈, 吉野一郎
  • Organizer: 第111回日本外科学会学術集会