Detection of gastrointestinal cancer biomarkers by next-generation mass spectrometry and comprehensive gene expression analysis

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390320
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto University (2013); University of Toyama (2011-2012)
• Principal Investigator: SHIMADA Yutaka 京都大学, 薬学研究科(研究院), 准教授 (30216072)
• Co-Investigator(Kenkyū-buntansha): TSUKADA Kazuhiro 富山大学, 大学院・医学薬学研究部, 教授 (90171967) ; OKUMURA Tomoyuki 富山大学, 大学院・医学薬学研究部, 助教 (10533523) ; ARIMA Miwako 埼玉県立がんセンター, 臨床腫瘍研究所, 副部長 (00312950) ; ABE Tetsuya 愛知県がんセンター, 研究所, 医長 (90378092) ; MONMA Kumiko 東京都立駒込病院, 内視鏡科部長 (70509471)
• Co-Investigator(Renkei-kenkyūsha): TSUJIMOTO Gozo 京都大学, 薬学研究科(研究院), 教授 (80172013)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: バイオマーカー / 食道癌 / miRNA

Research Abstract

We here evaluated a next-generation mass spectrometer and found that it not suitable for a comprehensive protein analysis of blood. Therefore, we performed a microRNA analysis of blood. To exclude individual differences between patients, we focused on esophageal mucosal cancer patients who were scheduled to undergo endoscopic mucosal resection (EMR) in the present study. Forty-three patients were recruited from 3 institutes. We analyzed the expression of miRNAs in pre-treatment as well as post-treatment blood samples that were collected from 31 patients. Significant differences were observed in the expression of 11 miRNAs between pre-treatment and post-treatment samples. We will analyze these miRNAs in 30 patients with advanced esophageal cancer in a future study and evaluate their efficacy as biomarkers of esophageal cancer.

Research Products

• [Journal Article] Expression analysis of fibroblast growth factor receptor-like 1 (FGFRL1) in esophageal squamous cell carcinoma (2014)
  • Author(s): Shimada Y, Okumura T, Nagata T, Hashimoto I, Sawada S, Y oshida T, Fukuoka J, Shimizu K, Tsukada K
  • Journal Title: Esophagus Volume: 11 Pages: 48-53
• [Journal Article] Relapse-associated microRNA in gastric cancer patients after S-1 adjuvant chemotherapy (2014)
  • Author(s): Omura T, Shimada Y, Nagata T, Okumura T, Fukuoka J, Y amagishi F, Tajika S, Nakajima S, Kawabe A, Tsukada K
  • Journal Title: Oncol Rep Volume: 31 Pages: 613-618
• [Journal Article] MicroRNA-203 inhibits the progression of esophageal squamous cell carcinoma with restored epithelial tissue architecture in vivo (2014)
  • Author(s): Okumura T, Shimada Y, Moriyama M, Takei Y, Omura T, Sekine S, Nagata T, Shimizu K2, Tsukada K
  • Journal Title: Int J Oncol Volume: 44 Pages: 1923-32
• [Journal Article] Possible involvement of glucose transporter 3 and 4 in esophageal leiomyoma with unusual high uptake of fluorine-18-fluorodeoxyglucose (2013)
  • Author(s): Shimada Y, Okumura T, Hori R, Moriyama M, Sekine S, Nagata T, Nomoto K, Fukuoka J, Tazawa K, Tsukada K
  • Journal Title: Three case reports- Esophagus Volume: 10 Pages: 34-41
• [Journal Article] Clinicopathological significance of deoxycytidine kinase expression in esophageal squamous cell carcinoma (2013)
  • Author(s): Shimada Y, Okumura T, Sekine S, Moriyama M, Hojo S, Matsui K, Sawada S, Nagata T, Fukuoka J, Tsukada K
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Pages: 716-720
• [Journal Article] Barrett' s adenocarcinoma arising 30 years after a Nissen fundoplication (2013)
  • Author(s): Shimada Y, Okumura T, Hashimoto I, Matsui K, Shibuya K, Hojo S, Nagata T, Hayashi S, Tsukada K
  • Journal Title: Esophagus Volume: 10 Pages: 179-183
• [Journal Article] Critical role for the receptor tyrosine kinase EPHB4 in esophageal cancers (2012)
  • Author(s): Hasina R, Mollberg N, Kawada I, Mutreja K, Kanade G, Y ala S, Surati M, Liu R, Li X, Zhou Y, Ferguson BD, Nallasura V, Cohen KS, Hyjek E, Mueller J, Kanteti R, El Hashani E, Kane D, Shimada Y, Lingen MW, Husain AN, Posner MC, Waxman I, Villaflor VM, Ferguson MK, V articovski L, V okes EE, Gill P, Salgia R
  • Journal Title: Cancer Res Volume: 73 Pages: 184-194
• [Journal Article] Expression Analysis of iPS Cell Inductive Genes in Esophageal Squamous Cell Carcinoma by Tissue Microarray (2012)
  • Author(s): Shimada Y, Okumura T, Sekine S, Moriyama M, Sawada S, Matsui K, Y oshioka I, Hojo S, Y oshida T, Nagata T, Fukuoka J, Tsukada K
  • Journal Title: Anticancer Res Volume: 32 Pages: 5507-5514
• [Journal Article] MicroRNA-210 Regulates Cancer Cell Proliferation through Targeting Fibroblast Growth Factor Receptor-Like 1 (FGFRL1) (2011)
  • Author(s): Tsuchiya S, Fujiwara T, Sato F, Shimada Y, Tanaka E, Sakai Y, Shimizu K, Tsujimoto G
  • Journal Title: J Biol Chem Volume: 286 Pages: 420-428
• [Journal Article] Polo-like Kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer (2011)
  • Author(s): Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru A V, Hamilton JP, Selaru FM, Y ang J, Matsumura N, Shimizu K, Abraham JM, Shimada Y, Mori Y, Meltzer SJ
  • Journal Title: Int J Cancer Volume: 129 Pages: 2134-2146
• [Journal Article] MicroRNA-141 Confers Resistance to Cisplatin-induced Apoptosis by Targeting Y AP1 in Human Esophageal Squamous Cell Carcinoma (2011)
  • Author(s): Imanaka Y, Tsuchiya S, Sato F, Shimada Y, Shimizu K, Tsujimoto G
  • Journal Title: J. Hum. Genet Volume: 56 Pages: 270-276
• [Journal Article] MicroRNA profiling of gastric cancer patients from formalin-fixed parafifin-embedded samples (2011)
  • Author(s): Osawa S, Shimada Y, Sekine S, Okumura T, Nagata T, Fukuoka J, Tsukada K
  • Journal Title: Oncology letters Volume: 2 Pages: 613-619
• [Presentation] Successful enucleation of esophageal leiomyoma in the prone position (2013)
  • Author(s): Shimada Y , Okumura T, Matsui K, Yoshioka I, Sawada S, Yoshida T , Nagata T , Tsukada K
  • Organizer: International surgical week ISW 2013
  • Place of Presentation: Helsinki
  • Year and Date: 2013-08-27
• [Presentation] Expression analysis of fibroblast growth factor receptor like 1 (FGFRL1) in esophageal cancer (2013)
  • Author(s): Shimada Y , Okumura T Nagata T , Hashimoto I, Sawada S, Y oshida T , Shimizu K, Tsukada K
  • Organizer: 48th Congress of European Society for Surgical Research
  • Place of Presentation: Istanbul
  • Year and Date: 2013-05-30
• [Presentation] Newly established Barrett' s adenocarcinoma cell line (TYAE-1) (2013)
  • Author(s): Shimada Y, Moriyama M, Okumura T, Kekine S, Sawada S, Matsui K, Hojo S, Shibuya K, Y oshioka I, Y oshida T , Nagata T , Tsukada T
  • Organizer: Annual meeting of American Association for Cancer Research
  • Place of Presentation: Washington DC
  • Year and Date: 2013-04-09
• [Presentation] Possible involvement of glucose transporter 3 and 4 in esophageal leiomyoma with unusual high uptake of fluorine-18-fluorodeoxyglucose (2012)
  • Author(s): Shimada Y , Okumura T, Hori R, Moriyama M, Sekine S, Nagata T , Nomoto K, Fukuoka J, Tazawa K, Tsukada K
  • Organizer: The 2012 ISDE congress
  • Place of Presentation: Venice (Italy)
  • Year and Date: 2012-10-16
• [Presentation] Identification of microRNAs which regulate stem cell property in esophageal squamous cell carcinoma (2012)
  • Author(s): Okumura T , Shimada Y, Sekine S, Moriyama M, Omura T , Kamiyama K, Hori R, Watanabe T , Hojo S, Matsui K, Y oshioka I, Sawada S, Y oshida T , Nagata T , Tsukada K
  • Organizer: The 2012 ISDE congress
  • Place of Presentation: Venice (Italy)
  • Year and Date: 2012-10-16
• [Presentation] Clinicopathological significance of deoxycytidine kinase expression in esophageal squamous cell carcinoma (2012)
  • Author(s): Shimada Y , Okumura T, Sekine S, Moriyama M, Hojo S, Sawada S, Y oshioka I, Nagata T , Fukumoto J, Tsukada K
  • Organizer: 47th ESSR Annual Congress
  • Place of Presentation: Lille (France)
  • Year and Date: 2012-06-08
• [Presentation] Identification of microRNAs which are differentially expressed in p75NTR-positive esophageal squamous cell carcinoma (2011)
  • Author(s): Okumura T , Shimada Y, Sekine S, Moriyama M, Omura T , Kamiyama K, Hori R, Watanabe T , Hojo S, Matsui K, Y oshioka I, Sawada S, Y oshida T , Nagata T , Tsukada K
  • Organizer: International surgical week 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-08-30
• [Presentation] Micro-RNA profiling of esophageal squamous cell cancer and gastric cancer from formalin-fixed paraffin -embedded (FFPE) specimens (2011)
  • Author(s): Shimada Y , Okumura T, Osawa S, Sawada S, Nagata T , Fukuoka J, Tsukada K
  • Organizer: 13th world congress on gastrointestinal cancer
  • Place of Presentation: Barcelona
  • Year and Date: 2011-06-23