Clarification and control on mechanism of neurogenesis of fluorescent enteric neurons.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23390330
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nara Medical University
• Principal Investigator: TAKAKI Miyako 奈良県立医科大学, 医学部, 特任教授 (00033358)
• Co-Investigator(Kenkyū-buntansha): KUNIYASU Hiroki 奈良県立医科大学, 医学部, 教授 (00253055)
• Co-Investigator(Renkei-kenkyūsha): NABEKURA Junichi 生理学研究所 (50237583) ; NAKAI Junichi 埼玉大学 (80237198)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: セロトニン４受容体 / 腸壁内神経 / 神経再生・新生 / 腸管切離吻合モデル / ２光子顕微鏡 / GCaMPトランスジェニックマウス

Outline of Final Research Achievements

The reconstruct of impaired enteric neural circuits at the thick granulation tissue at anastomosis in small intestine of H-line: Thy1 promoter GFP mouse was observed by two-photon microscopy (2PM) a week after drinking a 5-HT4-receptor agonist, mosapride citrate (MOS) 100 microM solution.
In Thy1-promoter YFP mouse after gut transection and anastomosis, the fetal brain-derived neural stem cells (NSC) with red fluorescent cell linker were transplanted from the tail vein. 5-HT4 receptor-mediated facilitation of neurogenesis was confirmed by clear three-dimensional in vivo imaging of newborn enteric neurons generated from enteric neural progenitors (host NSC; green fluorescence) and those from transplant NSC (red fluorescence). The facilitating effect of MOS and the distribution of new neurons were similar between the transplant and host NSC.
Finally, though preliminary, we succeeded in in vivo Ca2+ imaging of myenteric neuronal activity in the normal ileum of GCaMP6 transgenic mouse.

Free Research Field

生理学