Experimental study to repair non-heart beating donor lung using ex-vivo lung perfusion for clinical application

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390333
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: DATE Hiroshi 京都大学, 医学(系)研究科(研究院), 教授 (60252962)
• Co-Investigator(Kenkyū-buntansha): 小池 薫 京都大学, 医学(系)研究科 (10267164) ; 板東 徹 京都大学, 医学(系)研究科 (20293954) ; 阪井 宏彰 京都大学, 医学(系)研究科 (50362489) ; 陳 豊史 京都大学, 医学(系)研究科 (00452334) ; 佐藤 寿彦 京都大学, 医学(系)研究科 (40388822)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 心停止ドナー肺 / 肺移植

Research Abstract

One of the main obstacles in performing cadaveric lung transplantation from brain dead donor is shortage of available donor, especially in Japan. The purpose of this experimental animal study was to establish a clinically applicable method for lung transplantation utilizing non-heart-beating donors. The donor lungs obtained after cardiac arrest without heparinization were repaired by ex-vivo lung perfusion (EVLP) and were implanted to the recipient animal. Three experimental studies were conducted during 2011-13. 1) EVLP provided better lung preservation than cold static preservation using a canine left lung transplantation model in 2011. 2) Hypothermic EVLP repaired warm ischemic lung injury in a rat perfusion model in 2012. 3) Short term hypothermic EVLP repaired warm ischemic lung injury in a canine left lung transplantation model in 2013.

Research Products

• [Journal Article] Protective effect of surfactant inhalation against warm ischemic injury in an isolated rat lung ventilation model (2013)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Kobayashi M, Bando T, Date H
  • Journal Title: PLoS One Volume: 8(8) Pages: e72574
  • DOI: 10.1371/journal.pone.0072574
• [Journal Article] Protective effect of plasmin in marginal donor lungs in an ex vivo lung perfusion model (2013)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 32(5) Pages: 505-10
  • DOI: 10.1016/j.healun.2013.02.007
• [Journal Article] Reconditioning lungs donated after cardiac death using short-term hypothermic machine perfusion (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: Transplantation 2012 Volume: 94(10) Pages: 999-1004
  • DOI: 10.1097/TP.0b013e31826f632e
• [Journal Article] Therapeutic Effect of Surfactant Inhalation during Warm Ischemia in an Isolated Rat Lung Perfusion Model (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Yamada T, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transpl Int Volume: 25(10) Pages: 1096-105
  • DOI: 10.1111/j.1432-2277.2012.01532.x
• [Journal Article] The effect of beta-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Zhao X, Sakai H, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31(7) Pages: 773-9
• [Journal Article] Reconditioning of The Lungs from Non-Heart-Beating Donors with Normothermic Ex Vivo Lung Perfusion (2012)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Ohsumi A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31(2) Pages: 187-93
• [Journal Article] Effect of pre-procurement ventilation on lungs donated after cardiac death in a canine lung transplantation model (2011)
  • Author(s): Sakamoto J, Chen F, Yamada T, Nakajima D, Ohsumi A, Kikuchi R, Zhao X, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 864-70
• [Journal Article] Hypothermic machine perfusion ameliorates ischemia-reperfusion injury in rat lungs from non-heart-beating donors (2011)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Osumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 858-63
• [Presentation] Reconditioning of lungs donated after cardiac deathwith short-term hypothermic machine perfusion (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: CO10.02 XXIV International Congress of The Transplantation Society Berlin
  • Place of Presentation: Germany
  • Year and Date: 20120716-19
• [Presentation] Protective effect of plasmin for the non-heparinized donor lungs after cardiac death (2012)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: PO10.36 XXIV International Congress of The Transplantation Society Berlin
  • Place of Presentation: Germany
  • Year and Date: 20120716-19
• [Presentation] Prophylactic Surfactant Inhalation Attenuates Warm Ischemic Injury in Rat Lungs from Donation after Cardiac Death Donors (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Kobayashi M, Yamada T, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Organizer: CO33.08 XXIV International Congress of The Transplantation Society Berlin
  • Place of Presentation: Germany
  • Year and Date: 20120716-19
• [Presentation] Beta2-ADRENORECEPTOR AGONIST INHALATION AMELIORATES ISCHEMIA REPERFUSION INJURY FOLLOWING LUNG TRANSPLANTATION FROM NON-HEART-BEATING DONORS IN A CANINE MODEL. PB 5-2 (2011)
  • Author(s): Jin Sakamoto, Fengshi Chen, Daisuke Nakajima, Tetsu Yamada, Akihiro Ohsumi, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Toru Bando, Hiroshi Date
  • Organizer: The 12th Congress of the Asian Society of Transplantation
  • Place of Presentation: Seoul
  • Year and Date: 20110926-28
• [Presentation] Prophylactic surfactant inhalation ameliorates ischemia-reperfusion injury in rat lungs from donation after cardiac death donors. OB 5-3 (2011)
  • Author(s): Akihiro Ohsumi, Fengshi Chen, Daisuke Nakajima, Jin Sakamoto, Tetsu Yamada, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Toru Bando, Hiroshi Date
  • Organizer: The 12th Congress of the Asian Society of Transplantation
  • Place of Presentation: Seoul
  • Year and Date: 20110926-28