2013 Fiscal Year Final Research Report
Tumor-selective high LET and high RBE particles can overcome the radiation-resistant glioma stem cells
| Project/Area Number |
23390355
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWABATA Shinji 大阪医科大学, 医学部, 講師 (20340549)
MICHIUE Hiroyuki 岡山大学, 医歯薬総合研究科, 助教 (20572499)
KUROIWA Toshihiko 大阪医科大学, 医学部, 教授 (30178115)
KAJIMOTO Yoshinaga 大阪医科大学, 医学部, 教授 (30224413)
KIRIHATA Mitsunori 大阪府立大学, 21世紀科学研究機構, 教授 (60128767)
MASUNAGA Shin-ichirou 京都大学, 原子炉実験所, 教授 (80238914)
ONO Koji 京都大学, 原子炉実験所, 教授 (90122407)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | high LET / BNCT / glioma stem cell / 放射線抵抗性 / 癌幹細胞 |
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| Research Abstract |
GSLC were induced from GBM cell line A172. The phenotypes of GSLCs were confirmed by stem cell markers. These cells were irradiated with 60Co gamma rays or reactor neutron beams. Under neutron beam irradiation, high-LET proton particles can be produced. Radiosensitivity was assessed by a CFA, and the DNA double-strand breaks (DSBs) were assessed by gamma-H2AX focus assay. In stem cell culture medium, GSLCs could form neurosphere-like cells and express neural stem cell markers, Sox2 and Musashi, abundantly in comparison with their parental cells. GSLCs were significantly more radioresistant to gamma rays than their parental cells, but neutron beams overcame this resistance. Twenty-four hours after irradiation with gamma rays, there were significantly fewer gamma-H2AX foci in A172 GSLCs than in their parental cultured cells, while there was no apparent difference following neutron beam irradiation. High-LET radiation can overcome the radioresistance of GSLCs.
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