2013 Fiscal Year Final Research Report
Treatment strategy and analysis of progression mechanism of musculoskeletal tumors using fluorescent imaging
| Project/Area Number |
23390360
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Norio 金沢大学, 医薬保健学総合研究科, 特任教授 (90332668)
SHIRAI Toshiharu 京都府立医科大学, 大学院・医学研究科, 講師 (20397186)
HAYASHI Katsuhiro 金沢大学, 大学病院, 助教 (80507054)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 骨・軟部腫瘍学 |
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| Research Abstract |
Fluorescent labeled osteosarcoma cell line was transplanted in nude mice and harvested in lung metastasis to obtain higher metastatic cell line. The more metastatic potential, the more PAI -1 and u-PA levels increased. We previously reported that caffeine-assisted chemotherapy. We found caffeine affects tumor cells through various pathways, such as PTEN, AKT, NF-kB, and ERK activities. Modulation of cisplatinum efficacy induced by caffeine was visualized at the subcellular level by fluorescent ubiquitination-based cell cycle indicator in the nucleus. Caffeine overcame the cell-cycle arrest induced by cisplatinum, thereby increasing its efficacy. Theobromine, a caffeine derivative, was investigated as a new anti-cancer effect. It elevates intracellular cAMP levels and inhibits cell proliferation. Surgical navigation for bone and soft tissue tumor in mouse and human was also performed using ICG and near-infrared fluorescent imaging.
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[Journal Article] Theobromine, the primary methylxanthine found in Theobroma cacao, prevents malignant glioblastoma proliferation by negatively regulating phosphodiesterase-4, extracellular signal-regulated kinase, Akt/mammalian target of rapamycin kinase, and nuclear factor-kappa B2014
Author(s)
Sugimoto N, Miwa S, Hitomi Y, Nakamura H, Tsuchiya H, Yachie A
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Journal Title
Nutr Cancer
Volume: 66(3)
Pages: 419-23
DOI
Peer Reviewed
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[Journal Article] A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery2014
Author(s)
Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S, Yoshioka K, Inoue K, Ota T, Shinmura K, Tsuchiya H
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Journal Title
PLoS One
Volume: 9(2)
Pages: e88874
DOI
Peer Reviewed
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[Journal Article] Imaging UVC-induced DNA damage response in models of minimal cancer2013
Author(s)
Miwa S, Yano S, Hiroshima Y, Tome Y, Uehara F, Mii S, Efimova EV, Kimura H, Hayashi K, Tsuchiya H, Hoffman RM
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Journal Title
J Cell Biochem
Volume: 114(11)
Pages: 2493-9
DOI
Peer Reviewed
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[Journal Article] Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy2013
Author(s)
Miwa S, Yano S, Tome Y, Sugimoto N, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Efimova EV, Fujiwara T, Tsuchiya H, Hoffman RM
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Journal Title
J Cell Biochem
Volume: 114(11)
Pages: 2454-60
DOI
Peer Reviewed
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[Journal Article] Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells2013
Author(s)
Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV, Hoffman RM
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Journal Title
Anticancer Res
Volume: 33
Pages: 1373-7
Peer Reviewed
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[Journal Article] Diagnosis and treatment of low-grade osteosarcoma : experience with nine cases2013
Author(s)
Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Inatani H, Okamoto H, Yamada S, Ikeda H, Sawada-Kitamura S, Nojima T, Ooi A, Otsuka T
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities2013
Author(s)
Shimozaki S, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Kato T, Tsuchiya H
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Journal Title
J Orthop Sci
Volume: 19(1)
Pages: 156-63
DOI
Peer Reviewed
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[Journal Article] Prognostic value of histological response to chemotherapy in osteosarcoma patients receiving tumor-bearing frozen autograft2013
Author(s)
Miwa S, Takeuchi A, Ikeda H, Shirai T, Yamamoto N, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Igarashi K, Tsuchiya H
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Journal Title
PLoS One
Volume: 8(8)
Pages: e71362
DOI
Peer Reviewed
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[Journal Article] Histological study on liquid nitrogen-treated tumor-bearing bone of the proximal femur excised eight years after transplantation2012
Author(s)
Yamamoto N, Inatani H, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Shimozaki S, Kato T, Tsuchiya H
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Journal Title
Low Temp Med
Volume: 38
Pages: 5-8
Peer Reviewed
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[Journal Article] A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma2012
Author(s)
Miwa S, Taki J, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Ooi A, Tsuchiya H
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Journal Title
Journal of Surgical Oncology
Volume: 106
Pages: 273-9
DOI
Peer Reviewed
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[Journal Article] Caffeine induces apoptosis by inhibiting Akt/mTOR/S6K, NF-κB and MAPK pathways in osteosarcoma cell2012
Author(s)
Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A, Tsuchiya H
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Journal Title
Anticancer Research
Volume: 32
Pages: 3643-9
Peer Reviewed
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[Journal Article] Comparison of Cancer-Cell Seeding, Viability and Deformation in the Lung, Muscle and Liver, Visualized by Subcellular Real-time Imaging in the Live Mouse2011
Author(s)
Hayashi K, Kimura H, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Hasegawa A, Bouvet M, Hoffman RM
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Journal Title
Anticancer Res
Volume: 31
Pages: 3665-72
Peer Reviewed
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[Presentation] 骨腫瘍2013
Author(s)
土屋弘行
Organizer
日本小児整形外科学会
Place of Presentation
京都府立医科大学青蓮会館(京都)
Year and Date
20130824-25
Invited
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[Presentation] 腫瘍外科医の挑戦2011
Author(s)
土屋弘行
Organizer
第20回近整会夏季研究会(招待講演)
Place of Presentation
ホテルシーガルてんぽーざん大阪(大阪府)
Year and Date
2011-08-27
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