2013 Fiscal Year Final Research Report

Novel strategy for muscloskeletal diseases using secreted microRNA

Research Project

Project/Area Number	23390367
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Hiroshima University
Principal Investigator	ADACHI Nobuo 広島大学, 大学病院, 准教授 (30294383)
Co-Investigator(Kenkyū-buntansha)	OCHI Mitsuo 広島大学, 大学院・医歯薬保健学研究院, 教授 (70177244) KAMEI Naosuke 広島大学, 病院, 病院助教 (70444685) NAKASA Tomoyuki 広島大学, 病院, 病院助教 (60467769)
Project Period (FY)	2011-11-18 – 2014-03-31
Keywords	microRNA / non coding RNA / 尿 / 血清 / 運動器
Research Abstract	The purpose of this study was to examine whether secreted microRNA (miRNA) in urine from osteoarthritis (OA) patients could be a novel diagnostic marker for OA. Three healthy subjects, 6 low grade OA patients and 6 severe OA patients were included. Vesicle was isolated from urine by ultra-centrifugation, and quantity of vesicle and miRNAs in urine were identified using microarray. Severe OA urine had large amount vesicle compared to healthy and low grade OA. miRNA-16, 24, 25 and let-7b in urine from low grade OA were highly expressed compared to healthy and severe OA. These results suggested that urine miRNA could be a novel diagnostic marker for OA diagnosis.

(1 results)