2013 Fiscal Year Final Research Report
Novel strategy for muscloskeletal diseases using secreted microRNA
| Project/Area Number |
23390367
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
ADACHI Nobuo 広島大学, 大学病院, 准教授 (30294383)
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| Co-Investigator(Kenkyū-buntansha) |
OCHI Mitsuo 広島大学, 大学院・医歯薬保健学研究院, 教授 (70177244)
KAMEI Naosuke 広島大学, 病院, 病院助教 (70444685)
NAKASA Tomoyuki 広島大学, 病院, 病院助教 (60467769)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | microRNA / non coding RNA / 尿 / 血清 / 運動器 |
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| Research Abstract |
The purpose of this study was to examine whether secreted microRNA (miRNA) in urine from osteoarthritis (OA) patients could be a novel diagnostic marker for OA. Three healthy subjects, 6 low grade OA patients and 6 severe OA patients were included. Vesicle was isolated from urine by ultra-centrifugation, and quantity of vesicle and miRNAs in urine were identified using microarray. Severe OA urine had large amount vesicle compared to healthy and low grade OA. miRNA-16, 24, 25 and let-7b in urine from low grade OA were highly expressed compared to healthy and severe OA. These results suggested that urine miRNA could be a novel diagnostic marker for OA diagnosis.
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