2013 Fiscal Year Final Research Report
Aberrant DNA methylation of imprinted loci in sperm from infertile men and the causal mechanism
| Project/Area Number |
23390385
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
ARIMA Takahiro 東北大学, 医学(系)研究科(研究院), 教授 (80253532)
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| Co-Investigator(Kenkyū-buntansha) |
OKAE Hiroaki 東北大学, 大学院・医学系研究科, 助教 (10582695)
HIURA Hitoshi 東北大学, 大学院・医学系研究科, 助教 (70451523)
MIYAUCHI Naoko 東北大学, 大学院・医学系研究科, 技術補佐員 (60596162)
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| Co-Investigator(Renkei-kenkyūsha) |
YAEGASHI Nobuo 東北大学, 大学院・医学系研究科, 教授 (00241597)
NAKAI Kunihiko 東北大学, 大学院・医学系研究科, 教授 (00291336)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 生殖医学 / ゲノムインプリンティング |
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| Research Abstract |
Recent studies indicate the possibility of excess imprinting disease occurrence in babies by assisted reproductive technologies (ART). Genomic imprinting is regulated with epigenetic DNA methylation and the allele-specific expression of certain genes. In this study, we examined the DNA methylation status of 22 imprinted genes by bisulphite-based methylation analyses in sperm DNA from male ART patients. We found 43 patients (25.4%) whose sperm had abnormal DNA methylation among the sperm samples of men from 197 infertile couples. In addition, we found that abnormal spermatogenesis (leading to low sperm counts) is associated with defective methylation. These results would be of value in determining the safest and most ethical approach to use in ART.
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Research Products
(24 results)
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[Journal Article] Stability of the abnormal imprinting of human induced pluripotent stem cells2013
Author(s)
Hiura H, Toyoda M, Okae H, Sakurai M, Miyauchi N, Sato A, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Nishino K, Umezawa A, Arima T
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Journal Title
BMC Genetics
Volume: 14
Pages: 32
DOI
Peer Reviewed
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[Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproductive technologies2012
Author(s)
Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Van De PetteM, John R M, KagamiM, Nakai K, Soejima H, Ogata T, Arima T
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Journal Title
Human Reproduction
Volume: 27 (8)
Pages: 2541-2548
DOI
Peer Reviewed
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[Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method2012
Author(s)
Hiura H, Okae H, KobayashiH, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T
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Journal Title
BMC Medical Genomics
Volume: 5
Pages: 8-17
DOI
Peer Reviewed
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[Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus2011
Author(s)
Watanabe T, Tomizawa S, Mitsuya K, Totoki Y, Yamamoto Y, Kuramochi-Miyagawa S, Iida N, Hoki Y, Murphy P.J, Toyoda A, Gotoh K, Hiura H, Arima T, Fujiyama A, Sado T, Shibata T, Nakano T, Lin H, Ichiyanagi K, Soloway P.D. & Sasaki H
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Journal Title
Science
Volume: 332
Pages: 848-852
DOI
Peer Reviewed
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[Book] 卵子学2011
Author(s)
有馬隆博, 樋浦仁, 岡江寛明, 佐藤晶子, 宮内尚子
Total Pages
122-131
Publisher
京都大学学術出版会
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