Development of new treatment modality for daibetic macular edema using animal models

2014 Fiscal Year Final Research Report

• Project/Area Number: 23390400
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Shinshu University
• Principal Investigator: MURATA Toshinori 信州大学, 学術研究院医学系, 教授 (50253406)
• Co-Investigator(Kenkyū-buntansha): ISHIBASHI Tatsuro 九州大学, 大学院医学研究院, 教授 (30150428)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 黄斑浮腫 / 糖尿病 / 血管透過性亢進 / adrenomedullin / kockout mouse / VEGF

Outline of Final Research Achievements

Using two mouse model of retinal edema, i.e., receptor-activity-modifying protein (RAMP)-2 knockout mouse and kimba mouse which overexpresses vascular endothleial growth factor (VEGF) in the retina, we pursued the possibility for experimental treatment for macular edema. We investigated the effect of intraocular injection ofβ-catenin、PPARγ-ligand、apelin on retinal vascular leakage, but none of them exhibited prominent reducing effect of fluorescein dye leakage in flurorescein angiography. Then we investigated the effect of intravitreal and systemic injection of adrenomedullin on the hyperpermeability of retinal vessels in kimba mice. Adrenomedullin reduced fluorescein dye leakage from retinal vessels in kimba mice suggesting its therapeutic effect on retinal edema, in which VEGF plays a causative role. Since half-life of adrenomedullin is short, we consider that we have to synthesize a fusion protein of adrenomedullin and Fc portion of IgG to make its effect long lasting.

Free Research Field

眼科学　糖尿病網膜症