• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Annual Research Report

房水内生理活性物質が房水動態および濾過手術の成績に及ぼす影響に関する研究

Research Project

Project/Area Number 23390403
Research InstitutionKumamoto University

Principal Investigator

谷原 秀信  熊本大学, 大学院生命科学研究部, 教授 (60217148)

Co-Investigator(Kenkyū-buntansha) 井上 俊洋  熊本大学, 医学部附属病院, 講師 (00317025)
川路 隆博  熊本大学, 医学部附属病院, 講師 (30423677)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords緑内障 / サイトカイン / 房水
Research Abstract

濾過手術の成績を増悪させる要因として、手術既往眼や緑内障眼における房水中サイトカイン濃度の同時多発的な上昇が強く関与する可能性が示唆されていたため、その臨床的意義を解明し、房水流出路への生理的影響、緑内障眼における病的意義、そして濾過手術への副次的影響の検証を行うことを目指した。前年度までの解析によって、緑内障症例においてMCP-1の濃度が上昇しており、かつ絶対値も他のサイトカインと比較して高いことがわかっていた。
平成25年度は房水サンプル採取を行った症例についてその手術成績を追跡調査した。手術効果の検証はKaplan-Meier生存曲線およびCox hazards modelを用いた解析を行った。2回連続して設定眼圧を超えた場合、光覚を失った場合、眼圧下降のために追加手術を行った場合のいずれかを満たした時点を死亡と定義し、サイトカイン濃度の危険率を評価したところ、基準眼圧を21mmHgとした場合には房水MCP-1濃度が高い群においては低い群と比較して有意に手術成功率が低いことが判明し(P=0.018)、Cox hazards modelによる多変量解析においても房水MCP-1濃度は有意な因子であった(P=0.043)。次にウサギをを用いて緑内障手術の動物モデルを作成し、術直後と術翌日に前房内にMCP-1を注入し、無処置のコントロールと眼圧の変化を比較したところ、有意差はみとめなかった。この原因を探るため、前房内のMCP-1濃度を経時的に測定したところ、注入6時間後にはMCP-1濃度が急激に下降し、12時間後には注入前のレベルまで下降してしまうことが分かった。したがって動物モデルを用いた解析による検証は困難であることが判明したが、臨床症例において房水MCP-1が手術成績に影響を与えることが明らかとなった。

Current Status of Research Progress
Reason

25年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

25年度が最終年度であるため、記入しない。

  • Research Products

    (22 results)

All 2014 2013 Other

All Journal Article (13 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Epithelial mesenchymal transition-like phenomenon in trabecular meshwork cells2014

    • Author(s)
      Takahashi E, Inoue T, Fujimoto T, Kojima S, Tanihara H
    • Journal Title

      Experimental Eye Research

      Volume: 118 Pages: 72-79

    • DOI

      10.1016/j.exer.2013.11.014

    • Peer Reviewed
  • [Journal Article] Monocyte chemotactic protein-1 level in the aqueous humour as a prognostic factor for the outcome of trabeculectomy2014

    • Author(s)
      Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Clinical and Experimental Ophthalmology

      Volume: 印刷中 Pages: 印刷中

  • [Journal Article] Trabeculectomy for open-angle glaucoma in phakic eyes vs in pseudophakic eyes after phacoemulsification: a prospective clinical cohort study2014

    • Author(s)
      Takihara Y, Inatani M, Ogata-Iwao M, Kawai M, Inoue T, Iwao K, Tanihara H
    • Journal Title

      JAMA Ophthalmology

      Volume: 132 Pages: 69-76

    • DOI

      10.1001/jamaophthalmol.2013.5605.

  • [Journal Article] The 5-year incidence of bleb-related infection and its risk factors following filtering surgeries with adjunctive mitomycin C: CBIITS 22014

    • Author(s)
      Yamamoto T, Sawada A, Mayama C, Araie M, Ohkubo S, Sugiyama K, Kuwayama Y, The Collaborative Bleb-related Infection Incidence and Treatment Study Group
    • Journal Title

      Ophthalmology

      Volume: 印刷中 Pages: 印刷中

  • [Journal Article] Tear fluid signs associated with filtration blebs as demonstrated by three-dimensional anterior segment optical coherence tomography2014

    • Author(s)
      Kojima S, Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Clinical Ophthalmology

      Volume: 印刷中 Pages: 印刷中

  • [Journal Article] Fornix-based vs limbal-based conjunctival flaps in trabeculectomy with mitomycin C in high-risk patients2014

    • Author(s)
      Kuroda U, Inoue T, Awai-Kasaoka N, Shobayashi K, Kojima S, Tanihara H
    • Journal Title

      Clinical Ophthalmology

      Volume: 印刷中 Pages: 印刷中

  • [Journal Article] Risk factors for choroidal detachment after trabeculectomy with mitomycin C2013

    • Author(s)
      Haga A, Inatani M, Shobayashi K, Kojima S, Inoue T, Tanihara H
    • Journal Title

      Clinical Ophthalmology

      Volume: 7 Pages: 1417-1421

    • DOI

      10.2147/OPTH.S46375

    • Peer Reviewed
  • [Journal Article] Changes in visual acuity and intra-ocular pressure following bleb-related infection: the Japan Glaucoma Society Survey of Bleb-related Infection Report 22013

    • Author(s)
      Yamamoto T, Kuwayama Y, Nomura E, Tanihara H, Mori K; Japan Glaucoma Society Survey of Bleb-related Infection
    • Journal Title

      Acta Ophthalmologica

      Volume: 91 Pages: 420-426

    • DOI

      10.1111/aos.12079.

  • [Journal Article] Rho-associated kinase inhibitors: a novel glaucoma therapy2013

    • Author(s)
      Inoue T, Tanihara H
    • Journal Title

      Progress in Retinal and Eye Research

      Volume: 37 Pages: 1-12

    • DOI

      10.1016/j.preteyeres.2013.05.002.

  • [Journal Article] Oxidative stress response signaling pathways in trabecular meshwork cells and their effects on cell viability2013

    • Author(s)
      Awai-Kasaoka N, Inoue T, Kameda T, Fujimoto T, Inoue-Mochita M, Tanihara H
    • Journal Title

      Molecular Vision

      Volume: 19 Pages: 1332-1340

  • [Journal Article] Phase 2 randomized clinical study of a Rho kinase inhibitor, K-115, in primary open-angle glaucoma and ocular hypertension2013

    • Author(s)
      Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Araie M; K-115 Clinical Study Group
    • Journal Title

      American Journal of Ophthalmology

      Volume: 156 Pages: 731-736

    • DOI

      10.1016/j.ajo.2013.05.016.

  • [Journal Article] Elevated levels of multiple biomarkers of Alzheimer's disease in the aqueous humor of eyes with open-angle glaucoma2013

    • Author(s)
      Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Investigative Ophthalmology and Visual Science

      Volume: 54 Pages: 5353-5358

    • DOI

      10.1167/iovs.13-12245.

  • [Journal Article] Prognostic factors in trabeculectomy with mitomycin C having history of previous glaucoma surgery2013

    • Author(s)
      Awai-Kasaoka N, Inoue T, Inatani M, Takihara Y, Ogata-Iwao M, Tanihara H
    • Journal Title

      Japanese Journal of Ophthalmology

      Volume: 57 Pages: 514-519

    • DOI

      10.1007/s10384-013-0257-5.

  • [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Secondary Glaucoma2013

    • Author(s)
      Inoue T, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      20130717-20130720
  • [Presentation] Visualization of actin filament using time-lapse fluorescent microscopy in trabecular meshwork cells2013

    • Author(s)
      Fujimoto T, Inoue T, Inoue-Mochita M, Kasaoka N, Shobayashi K, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      20130717-20130720
  • [Presentation] A short-term efficacy and safety of brimonidine 0.1% as fourth-line therapy in open-angle glaucoma subjects2013

    • Author(s)
      Hirakawa S, Kojima S, Inoue T, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      20130717-20130720
  • [Presentation] Short-term prospective investigation of filtering bleb by three dimensional anterior-segment optical coherence tomography2013

    • Author(s)
      Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      20130717-20
  • [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Secondary Glaucoma2013

    • Author(s)
      Inoue T, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Year and Date
      20130505-20130509
  • [Presentation] Short-term prospective investigation of filtering bleb by three dimensional anterior-segment optical coherence tomography2013

    • Author(s)
      Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Year and Date
      20130505-09
  • [Presentation] Identification of TGF-β2-induced proinflammatory cytokines secreted from cultured trabecular meshwork cells2013

    • Author(s)
      Inoue-Mochita M, Inoue T, Fujimoto T, Awai-Kasaoka N, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Year and Date
      20130505-09
  • [Presentation] Evaluation of filtering blebs with transconjunctival oozing by anterior segment optical coherence tomography2013

    • Author(s)
      Nakashima K, Inoue T, Fukushima A, Hirakawa S, Kawaji T, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Year and Date
      20130505-09
  • [Remarks] 熊本大学大学院生命科学研究部眼科学分野 研究業績

    • URL

      http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/gyouseki.html

URL: 

Published: 2015-05-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi