2013 Fiscal Year Final Research Report
Identification of master transcription factors in corneal epithelial cells
| Project/Area Number |
23390404
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KINOSHITA Shigeru 京都府立医科大学, 医学(系)研究科(研究院), 教授 (30116024)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Morio 京都府立医科大学, 医学研究科, 助教 (40426531)
KAWASAKI Satoshi 大阪大学, 医学研究科, 講師 (60347458)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 角膜上皮 / 転写因子 / ダイレクトリプログラミング / コア転写因子 / マスターレギュレーター |
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| Research Abstract |
We attempted to identify master transcription factors (TFs) in corneal epithelial cells (CECs) which are able to maintain the cell-type specific transcriptional profile. First, we used microarray analysis to select candidate master TFs in CECs from all the genes, which were highly expressed in CECs compared to various other cells. In order to narrow down the master TFs in CECs, iPS interference screening was performed and produced 20 candidate TFs. A particular combination of candidate TFs was able to induce CEC-like cells expressing keratin 12, which is a corneal specific marker when overexpressed in a heterologous cell type. Furthermore, the expression level of other corneal-related genes, such as keratin 3, keratin 15, aldehyde dehydrogenase 3, transketolase, and E-cadherin were upregulated in the induced cells. These results suggested that defined TFs, which enabled the induction of CECs, contributed to the maintenance of the corneal-specific transcriptional profile.
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[Journal Article] LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis2014
Author(s)
Nakamura T, Hamuro J, Takaishi M, Simmons S, Maruyama K, Zaffalon A, Bentley AJ, Kawasaki S, Nagata-Takaoka M, Fullwood NJ, Itami S, Sano S, Ishii M, Barrandon Y, Kinoshita S
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Journal Title
J Clin Invest
Volume: 124(1)
Pages: 385-397
Peer Reviewed
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[Journal Article] Visual improvement following cultivated oral mucosal epithelial transplantation2013
Author(s)
Sotozono C, Inatomi T, Nakamura T, Koizumi N, Yokoi N, Ueta M, Matsuyama K, Miyakoda K, Kaneda H, Fukushima M, Kinoshita S
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Journal Title
Ophthalmology
Volume: 120(1)
Pages: 193-200
Peer Reviewed
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[Presentation] Investigation of gene therapy using immortalized cells derived from a Gelatioous Drop-Like Dystrophy patient2013
Author(s)
Kitazawa K, Kawasaki S, Aoi K, Shinomiya K, Matsuda A, Funaki T, Nakatsukasa M, Murakami A, Kinoshita S
Organizer
The Association for Research in Vision and Ophthalmology, Seatle (ARVO) 2013
Place of Presentation
Seatle, USA
Year and Date
2013-05-08
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[Presentation] Integral Analysis of Gene Signatures and MicroRNA Expression of Cultured Human Corneal Endothelial Cells in Relation to Their Functions, Cell Senescence, Epithelial-Mesenchymal Transition, and Fibrosis2013
Author(s)
Asada K, Toda M, Hagiya M, Nakata K, Ueno M, Okumura N, Koizumi N, Hamuro J, Kinoshita S
Organizer
The Association for Research in Vision and Ophthalmology 2013
Place of Presentation
Seattle, USA
Year and Date
2013-05-07
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[Presentation] Transfer of TACSTD2 gene into corneal epithelial cells of Gelatinous drop like corneal dystrophy and its functional expression2013
Author(s)
Matsunaga T, Kitazawa K, Yamasaki K, Sato T, Watanabe Y, Funaki T, Matsuda A, Ebihara N, Kawasaki S, Murakami A
Organizer
The Association for Research in Vision and Ophthalmology, Seatle (ARVO) 2013
Place of Presentation
Seattle, USA
Year and Date
2013-05-07
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[Presentation] Efficacy and Safety Evaluation of Cell-Injection Therapy using Cultivated Human Corneal Endothelial Cells2013
Author(s)
Koizumi N, Okumura N, Shiina T, Suzuki S, Nakamura S, Sakamoto Y, Yamasaki K, Ueno M, Hamuro J, Kinoshita S
Organizer
The Association for Research in Vision and Ophthalmology 2013
Place of Presentation
Seattle, USA
Year and Date
2013-05-06
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[Presentation] Establishment of immortalized corneal and conjunctival epithelial cells lacking the functional TACSTD2 gene2012
Author(s)
Kitazawa K, Kawasaki S, Shinomiya K, Matsuda A, Funaki F, Yamasaki K, Nakatsukasa M, Fukuoka H, Ebihara N, Murakami A, Kinoshita S
Organizer
The Association for Research in Vision and Ophthalmology, Fort Lauderdale (ARVO) 2012
Place of Presentation
Ft. Lauderdale, USA
Year and Date
2012-05-07
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