Functional analysis of bone marrow-derived cells on control of the whole-body responses to injury/illness, regeneration and tissue restoration

2015 Fiscal Year Final Research Report

• Project/Area Number: 23390413
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Keio University
• Principal Investigator: NAMIKI JUN 慶應義塾大学, 医学部, 准教授 (20189195)
• Co-Investigator(Kenkyū-buntansha): ISHIHAMA YASUSHI 京都大学, 薬学研究科, 教授 (30439244)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 臨床 / 生体侵襲 / 蛋白質 / プロテオーム / 集中治療医学 / 単球 / 骨髄 / シグナル伝達

Outline of Final Research Achievements

In severe sepsis patients, the ratio of CD14・CD16-double positive activated monocytes to CD14-positive monocytes measured by a flow-cytometer correlated to the severity of sepsis: increase at an early phase and decrease with a recovery of the illness to the normal levels of the ratio. On the other hand, activated monocytes decreased in severe trauma and burn patients, especially CD14-positive monocyte almost disappeared in the peripheral blood of the most severe case, indicating that the biological responses were suppressed to the excessive severity of injuries.
Phospho-proteome analysis showed that a DAMPs-related protein was phosphorylated in monocytes obtained from the severe sepsis patients. This phosphorylation of the intracellular protein may be an early physiological response to sepsis.

Free Research Field

救急医学