2013 Fiscal Year Final Research Report
Visualization of functional dynamics in bone cells by three dimensional fluorescence imaging toward understanding of bone physiopathology
| Project/Area Number |
23390417
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Ehime University (2013)
Tokyo Medical and Dental University (2011-2012) |
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Principal Investigator |
IIMURA Tadahiro 愛媛大学, プロテオサイエンスセンター, 准教授(特定教員) (20282775)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | イメージング / 骨代謝 / 骨細胞 |
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| Research Abstract |
The purpose of this study is to unveil novel mechanisms of bone pathophysiology by exploiting three-dimensional (3D) fluorescence morphometry. After acquisition of fluorescence optical sections, 3D images were reconstructed in silico, by which. detailed distribution pattern and signal intensity of molecule of interest can be quantitatively analyzed. We successfully measured and compared osteocyte-network pattern in bones loaded by distinct physiological tensions. Spatio-temporal dynamics of Sclerostion production by osteocytes during postnatal bone development was described. Furthermore, we uncovered unique regulatory mechanism of FGF23 by DMP1.
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