2013 Fiscal Year Final Research Report
Molecular mechanism by which the alternative NF-kB pathway regulates bone metabolism and bone regeneration
| Project/Area Number |
23390424
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
JIMI EIJIRO 九州歯科大学, 歯学部, 教授 (40276598)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Kazuhiro 東京医科歯科大学, 歯学部, 准教授 (40272603)
KATAGIRI Takenobu 埼玉医科大学, 医学部, 教授 (80245802)
FUKUSHIMA Hidefumi 九州歯科大学, 九州歯科大学, 福岡歯科大学, 歯学部, 歯学部, 歯学部, 助教, 講師, 准教授 (70412624)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 骨芽細胞 / 破骨細胞 / NF-κB |
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| Research Abstract |
We investigated the molecular mechanisms triggered by the alternative NF-kB pathway in the regulation of osteoblast and osteoclast differentiation. Osteoblastic differentiation induced by BMP2, as shown by alkaline phosphatase activity. Smad1 phosphorylation was also enhanced in primary osteoblasts from aly/aly mice. The ectopic bone formation in vivo that was induced by BMP2 was enhanced in aly/aly mice compared with controls. On the other hand, the overexpression of RelB in aly/aly bone marrow cells rescues RANKL-induced osteoclastogenesis by inducing p100 processing. Cot, an MAP3K, was up-regulated by RelB overexpression and knocking down of Cot expression significantly reduced the RANKL-induced osteoclastogenesis induced by RelB overexpression. Thus, the alternative the NF-kB pathway via the processing of p52 from p100 negatively regulates osteoblastic differentiation by modifying BMP activity and positively regulates RANKL-induced osteoclastogenesis.
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Research Products
(24 results)
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[Journal Article] RelB-induced Expression of Cot, an MAP3K Family Member, Rescues RANKL-induced Osteoclastogenesis in Alymphoplasia Mice by Promoting NF-κB2 Processing by IKKα2014
Author(s)
Taniguchi R, Fukushima H, Osawa K, Maruyama T, Yasuda H, Weih F, Doi T, Maki K, Jimi E
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Journal Title
J Biol Chem
Volume: 289(11)
Pages: 7349-7361
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[Journal Article] Disruption of NF-κB1 prevents bone loss caused by mechanical unloading2013
Author(s)
Nakamura H, Aoki K, Masuda W, Alles N, Nagano K, Fukushima H, Osawa K, Yasuda H, Nakamura I, Mikuni-Takagaki Y, Ohya K, Maki K, Jimi E
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Journal Title
J Bone Miner Res
Volume: 28
Pages: 1457-67
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[Journal Article] Identification of a novel bone morphogenetic protein (BMP)-inducible transcript, BMP-inducible transcript-1, by utilizing the conserved BMP-responsive elements in the Id genes2013
Author(s)
Shin M, Ohte S, Fukuda T, Sasanuma H, Yoneyama K, Kokabu S, Miyamoto A. Tsukamoto S, Hohjoh H, Jimi E, Katagiri T
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Journal Title
J BoneMiner Metab
Volume: 31
Pages: 34-43
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[Journal Article] Accumulation of p100, a precursor of NF-κB2, enhances osteoblastic differentiation in vitro and bone formation in vivo in aly/aly mice2011
Author(s)
SeoY, Fukushima H, Maruyama T, Nakao Kuroishi K, Osawa K, Nagano K, Aoki K, Weih F, Doi T, Zhang M, Ohya K, Katagiri T, Hosokawa R, Jimi E
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Journal Title
Mol Endocrinol
Volume: 26
Pages: 414-422
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[Journal Article] Identification and functional analysis of Zranb2 as a novel Smad-binding protein that suppresses BMP signaling2011
Author(s)
Ohte S, Kokabu S, Iemura SI, Sasanuma H, Yoneyama K, Shin M, Suzuki S, Fukuda T, Nakamura Y, Jimi E, Natsume T, Katagiri T
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Journal Title
J Cell Biochem
Volume: 113
Pages: 808-814
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