2013 Fiscal Year Final Research Report
Dental pulp regeneration by stem cell transplantation: development of scaffolds and establishment of an animal experimental model
| Project/Area Number |
23390433
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIBA Nagako 新潟大学, 医歯学総合病院, 講師 (10323974)
YOSHIBA Kunihiko 新潟大学, 医歯学系, 准教授 (30220718)
OHSHIMA Hayato 新潟大学, 医歯学系, 教授 (70251824)
KANEKO Tomoatsu 新潟大学, 医歯学総合病院, 助教 (70345297)
SHIGETANI Yoshimi 新潟大学, 医歯学系, 助教 (80397132)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 歯学 / 再生医学 / 細胞•組織 / 歯髄 / スキャホールド / 幹細胞 |
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| Research Abstract |
This study was designed with the ultimate purpose of establishing a tooth pulp tissue engineering using transplantation of dental pulp stem cells, and conducted to select appropriate scaffolds and develop an experimental engineering system using rat molars. It was demonstrated that rat dental pulp is equipped with stem-like cells that coexpress CD146 and MAP1B and are distributed predominantly in theperivascular area. rat dental pulp by means of immunohistochemistry. Moreover, transplantation of rat mesenchymal stem cells into pulpotomized rat molars with a PLLA/Puramatrix scaffold resulted in the formation of new mineralized tissues at 4 weeks. These results suggest that the experimental model used in this study is useful for investigating the pulp tissue regeneratiopn using stem cell transplantation.
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