キナーゼ抗体ライブラリーを用いた新規口腔がん治療標的分子の探索

2015 Fiscal Year Annual Research Report

• Project/Area Number: 23390469
• Research Institution: National Cancer Center Japan
• Principal Investigator: 本田 一文 国立研究開発法人国立がん研究センター, その他部局等, その他 (10260936)
• Co-Investigator(Kenkyū-buntansha): 森 泰昌 国立研究開発法人国立がん研究センター, その他部局等, 研究員 (00296708) ; 関根 茂樹 国立研究開発法人国立がん研究センター, その他部局等, 研究員 (10321879) ; 平岡 伸介 国立研究開発法人国立がん研究センター, その他部局等, その他 (40276217) ; 渡部 幸央 東京歯科大学, 歯学部, その他 (50733490) ; 出雲 俊之 東京医科歯科大学, 歯学部附属病院, 准教授 (80322709)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: キノーム / 口腔がん / 抗体基盤プロテオミクス / 分子標的治療

Outline of Annual Research Achievements

口腔がんの分子標的治療薬剤のバリエーションは他のがん種に比べて少ない。現在、各種がん治療に利用される分子標的には、タンパク質リン酸化酵素（キナーゼ）が選択されることが多い。口腔がんにおけるキナーゼ発現プロファイルから、口腔がんに特有な新規分子標的治療法を開発できる可能性がある。そこで、キナーゼ抗体ライブラリーを用いて網羅的発現解析から口腔がんキナーゼプロファイルを完成させ、新規分子標的の可能性がある3個のキナーゼを同定した。そのうち１つは口腔がんの浸潤先進部に強く発現し、口腔がん転移阻止治療の開発に貢献する可能性を示した。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Efficacy of adjuvant chemotherapy for non-small cell lung cancer assesed by metastatic potential assocaited with ACTN4 (2016)
  • Author(s): Miura N, Kamita M, Kakuya T, Fujiwara Y, Tsuta K, Shiraishi H, Takeshita F, Ochiya T, Hirokazu Shoji, Huang W, Ohe Y, Yamada T, Honda K
  • Journal Title: Oncotarget Volume: Apr 21 Pages: 1-14
  • DOI: DOI: 10.18632/oncotarget.8890
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Patterns of recurrence after selective postoperative radiation therapy for patients with head and neck squamous cell carcinoma. (2016)
  • Author(s): Murakami N, Matsumoto F, Yoshimoto S, Ito Y, Mori T, Ueno T, Tuchida K, Kashihara T, Kobayashi K, Harada K, Kitaguchi M, Sekii S, Umezawa R, Takahashi K,
  • Journal Title: BMC Cancer. Volume: Mar 7;16(1): Pages: 192-202
  • DOI: doi: 10.1186/s12885-016-2229-x.
  • Peer Reviewed / Open Access
• [Journal Article] SMYD2 overexpression is associated with tumor cell proliferation and a worse outcome in human papillomavirus-unrelated nonmultiple head and neck carcinomas. (2016)
  • Author(s): Ohtomo-Oda R, Komatsu S, Mori T, Sekine S, Hirajima S, Yoshimoto S, Kanai Y, Otsuji E, Ikeda E, Tsuda H.
  • Journal Title: Hum Pathol. Volume: Mar;49: Pages: 145-55.
  • DOI: doi:10.1016/j.humpath.2015.08.025.
  • Peer Reviewed
• [Journal Article] CIC-rearranged Sarcomas: A Study of 20 Cases and Comparisons With Ewing Sarcomas. (2016)
  • Author(s): Yoshida A, Goto K, Kodaira M, Kobayashi E, Kawamoto H, Mori T, Yoshimoto S, Endo O, Kodama N, Kushima R, Hiraoka N, Motoi T, Kawai A.
  • Journal Title: Am J Surg Pathol. Volume: Mar;40(3): Pages: 313-23.
  • DOI: doi: 10.1097/PAS.0000000000000570
  • Peer Reviewed
• [Journal Article] Primary intraocular synovial sarcoma in the post retinal detachment operative state. (2016)
  • Author(s): Ito J, Suzuki S, Yoshida A, Mori T.
  • Journal Title: BMJ Case Rep Volume: Aug 6;2015 Pages: 1-2
  • DOI: doi: 10.1136/bcr-2015-209919.
  • Peer Reviewed / Open Access
• [Journal Article] A significant subgroup of resectable gallbladder cancer patients has an HER2 positive status. (2016)
  • Author(s): Yoshida H, Shimada K, Kosuge T, Hiraoka N
  • Journal Title: Virchows Arch. Volume: Apr;468(4): Pages: 431-9
  • DOI: 10.1007/s00428-015-1898-1. Epub 2016 Jan 13.
  • Peer Reviewed
• [Journal Article] Malignant transformation of oral leukoplakia with a focus on low-grade dysplasia (2016)
  • Author(s): Watabe Yukio, Nomura Takeshi, Onda Takeshi, Yakushiji Takashi, Yamamoto Nobuharu, Ohata Hitoshi, Takano Nobuo, Shibahara Takahiko
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 28 Pages: 2212-5558
  • DOI: doi:10.1016/j.ajoms.2015.02.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plasma biomarker for detection of early stage pancreatic cancer and risk factors for pancreatic malignancy using antibodies for apolipoprotein-AII isoforms. (2015)
  • Author(s): Honda K, Kobayashi M, Okusaka T, Rinaudo JA, Huang Y, Marsh T, Sanada M, Sasajima Y, Nakamori S, Shimahara M, Ueno T, Tsuchida A, Sata N, Ioka T, Yasunami Y, Kosuge T, Miura N, Kamita M, Sakamoto T, Shoji H, Jung G, Srivastava S, Yamada T.
  • Journal Title: Scientific Reports Volume: Nov 9;5 Pages: 15921
  • DOI: doi: 10.1038/srep15921
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The biological role of actinin-4 (ACTN4) in malignant phenotypes of cancer. (2015)
  • Author(s): Honda K
  • Journal Title: Cell Biosci. Volume: Aug 18;5 Pages: 41-49
  • DOI: 10.1186/s13578-015-0031-0.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clinical significance of tumor-infiltrating immune cells focusing on BTLA and Cbl-b in patients with gallbladder cancer. (2015)
  • Author(s): Oguro S, Ino Y, Shimada K, Hatanaka Y, Matsuno Y, Esaki M, Nara S, Kishi Y, Kosuge T, Hiraoka N.
  • Journal Title: Cancer Sci Volume: Dec;106(12) Pages: 1750-60
  • DOI: 10.1111/cas.12825. Epub 2015 Oct 22.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TERT promoter mutations are frequent and show association with MED12 mutations in phyllodes tumors of the breast. (2015)
  • Author(s): Yoshida M, Ogawa R, Yoshida H, Maeshima A, Kanai Y, Kinoshita T, Hiraoka N, Sekine S.
  • Journal Title: Br J Cancer Volume: Oct 20;113(8) Pages: 1244-8.
  • DOI: 10.1038/bjc.2015.326.
  • Peer Reviewed
• [Journal Article] Activating GNAS and KRAS mutations in gastric foveolar metaplasia, gastric heterotopia, and adenocarcinoma of the duodenum. (2015)
  • Author(s): Matsubara A, Ogawa R, Suzuki H, Oda I, Taniguchi H, Kanai Y, Kushima R, Sekine S.
  • Journal Title: Br J Cancer. Volume: Apr 14;112(8) Pages: 1398-404.
  • DOI: 10.1038/bjc.2015.104.
  • Peer Reviewed
• [Presentation] Plasma biomarker for detection of early-stage pancreatic cancer and risk factors for pancreatic malignancy using antibodies for apolipoprotein-A2 isoforms (2016)
  • Author(s): Kazufumi Honda, Michimoto Kobayashi, Takuji Okusaka, Chigusa Morizane, Jo Ann Rinaudo, Ying Huang, Tracey Marsh, Shoji Nakamori, Masashi Shimahara, Takaaki Ueno, Akihiko Tsuchida, Naohiro Sata, Tatsuya Ioka, Tomoo Kosuge, Yohichi Yasunami, Masaru Yoshida, Ken Takeshi Azuma, Sudhir i Srivastava, Tesshi Yamada
  • Organizer: American Society Clinical Oncology (ASCO) Annual Meeting 2016
  • Place of Presentation: Chicago USA
  • Year and Date: 2016-06-04 – 2016-06-07
• [Presentation] Plasma biomarker to detect early stage and risk disease of pancreatic cancer using apolipoprotein isoforms (2016)
  • Author(s): Honda K, Kobayashi M, Srivastava S.
  • Organizer: Biomarker Summit 2016
  • Place of Presentation: San Diago USA
  • Year and Date: 2016-03-21 – 2016-03-23
  • Int'l Joint Research
• [Presentation] Plasma biomarker for detecting early-stage pancreatic cancer and risk factors for pancreatic malignancy using antibodies for apolipoprotein-AII isoforms (2016)
  • Author(s): Honda K.
  • Organizer: Japan US Workshop for Cancer Reseach
  • Place of Presentation: Tokyo Japan
  • Year and Date: 2016-03-07 – 2016-03-08
  • Int'l Joint Research / Invited
• [Presentation] 遺伝子の変異と発現変調がおよぼす口腔癌の発症と進行 (2016)
  • Author(s): 森 泰昌
  • Organizer: 第３４回日本口腔腫瘍学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-01-21 – 2016-01-21
• [Presentation] Copy number of ACTN4 is a predictive indicator for late metastasis of cervical lymph nodes in early-stage tongue cancer (2015)
  • Author(s): Kakuya T, Mori T, Watabe Y, Yoshimoto S, Miura N, Nomura T, Huang W, Shibahara T, Honda K
  • Organizer: International Conference on Oral and Maxillofacial Surgery (ICOMS) 2016
  • Place of Presentation: Melbourne Australia
  • Year and Date: 2015-10-27 – 2015-10-30
  • Int'l Joint Research