Development of novel molecular targets for oral cancer with kinome profile by antibody-based proteomics

2015 Fiscal Year Final Research Report

• Project/Area Number: 23390469
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: National Cancer Center Japan
• Principal Investigator: Kazufumi Honda 国立研究開発法人国立がん研究センター, その他部局等, その他 (10260936)
• Co-Investigator(Kenkyū-buntansha): MORI Taisuke 国立研究開発法人国立がん研究センター, 中央病院, 医員 (00296708) ; Sekine Shigeki 国立研究開発法人国立がん研究センター, 中央病院, 医長 (10321879) ; HIRAOKA Nobuyoshi 国立研究開発法人国立がん研究センター, 中央病院, 科長 (40276217) ; WATABE Yukio 東京歯科大学, レジデント (50733490) ; IZUMO Toshiyuki 国立大学法人東京医科歯科大学, 准教授 (80322709)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: キノーム / 口腔がん / 分子標的治療

Outline of Final Research Achievements

Molecular target therapy has gained attention among the therapeutic strategies for cancer. To identify novel molecular targets for tongue cancer, we performed a proteomic analysis of surgical specimens of tongue cancer using an antibody library consisting of the kinome.
We created a tissue microarray (TMA) comprising tongue cancer, and then we screened overexpressing kinase proteins in tongue cancer by antibody library. We identified kinase-X, which was overexpressed in the invasive front of the tongue cancer, but was not expressed at the normal mucosa of the tongue. Overexpression resulted from transfection of kinase-X to tongue cancer cells that showed increased cell motility. The increased cell motility was decreased by an administration of a small molecular inhibitor of kinase-X. Proliferation of cells transfected with kinase-X was decreased by the inhibition of kinase-X. We concluded that kinase-X has potential as a novel molecular target for squamous cell carcinoma of the tongue.

Free Research Field

外科系歯学