2015 Fiscal Year Final Research Report
Development of novel molecular targets for oral cancer with kinome profile by antibody-based proteomics
Project/Area Number |
23390469
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | National Cancer Center Japan |
Principal Investigator |
Kazufumi Honda 国立研究開発法人国立がん研究センター, その他部局等, その他 (10260936)
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Co-Investigator(Kenkyū-buntansha) |
MORI Taisuke 国立研究開発法人国立がん研究センター, 中央病院, 医員 (00296708)
Sekine Shigeki 国立研究開発法人国立がん研究センター, 中央病院, 医長 (10321879)
HIRAOKA Nobuyoshi 国立研究開発法人国立がん研究センター, 中央病院, 科長 (40276217)
WATABE Yukio 東京歯科大学, レジデント (50733490)
IZUMO Toshiyuki 国立大学法人東京医科歯科大学, 准教授 (80322709)
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Project Period (FY) |
2011-04-01 – 2016-03-31
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Keywords | キノーム / 口腔がん / 分子標的治療 |
Outline of Final Research Achievements |
Molecular target therapy has gained attention among the therapeutic strategies for cancer. To identify novel molecular targets for tongue cancer, we performed a proteomic analysis of surgical specimens of tongue cancer using an antibody library consisting of the kinome. We created a tissue microarray (TMA) comprising tongue cancer, and then we screened overexpressing kinase proteins in tongue cancer by antibody library. We identified kinase-X, which was overexpressed in the invasive front of the tongue cancer, but was not expressed at the normal mucosa of the tongue. Overexpression resulted from transfection of kinase-X to tongue cancer cells that showed increased cell motility. The increased cell motility was decreased by an administration of a small molecular inhibitor of kinase-X. Proliferation of cells transfected with kinase-X was decreased by the inhibition of kinase-X. We concluded that kinase-X has potential as a novel molecular target for squamous cell carcinoma of the tongue.
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Free Research Field |
外科系歯学
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