2013 Fiscal Year Final Research Report
Investigaion of a new mechanism of growth factor signaling for regulation of neurogenesis
| Project/Area Number |
23500389
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Kyoto University |
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Principal Investigator |
SATO Tomomi 京都大学, 再生医科学研究所, 研究員 (50373311)
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| Co-Investigator(Kenkyū-buntansha) |
KURISAKI Tomohiro 埼玉医科大学, 医学部, 講師 (90311422)
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| Research Collaborator |
SATO Fuminori 京都大学, 再生医科学研究所, 研究員 (10588263)
SAKAGUCHI Kazuya 京都大学, 再生医科学研究所, 大学院生
TANIGOME Ryoma 京都大学, 再生医科学研究所, 大学院生
KAMEZAKI Aosa 京都大学, 再生医科学研究所, 大学院生
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| Project Period (FY) |
2011 – 2013
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| Keywords | 神経発生 / 増殖因子 / 視蓋 / ゼブラフィッシュ |
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| Research Abstract |
Brain development starts with neurogenesis from neural stem/radial glial (NS/RG) cells in the ventricular (V) region. RG cells not only self-renew in the V region, but also give rise to neural progenitor (NP) cells, which also self-renew and/or produce post-mitotic neurons in the subventricular (SV) region. However, little is known about regulatory mechanisms for the generation of neurons from NP cells.
Here, we showed that neuronal generation from NP cells is a spatially and temporally regulated process and depends on Neuregulin 1 (NRG1) using the optic tectum (OT) of zebrafish embryos as a model system. NRG1 regulates cell divisions of NP cells in the SV region. Rescue of NRG1-depleted embryos with recombinant human NRG1 suggested a conserved role for NRG1 as an extrinsic signal for neuron production. Moreover, live imaging showed that a removal of the ErbB inhibitor from embryos triggered neuron-generating divisions of NP cells in the SV region.
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