2013 Fiscal Year Final Research Report
Analysis of the mechanisms underlying the layer- and region-specificity during brain development
| Project/Area Number |
23500390
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 脳形態形成 / 神経幹細胞 / 神経発生 |
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| Research Abstract |
We analyzed the stage- and region-specific gene expression in neural stem cells during cortical development. We performed the functional analysis of Hbp1 that is highly expressed during the neurogenic stages, and found the abnormal brain morphogenesis including enlarged ventricles and thinner cortex in the Hbp1 conditional knockout mice. In the transgenic mice in which Hes1 expression is controlled by the Tet-On system, persistent high expression of Hes1 led to the maintenance of neural stem cells and the expansion of neural stem cell pool in embryonic, postnatal and adult brains. The number of Tbr2-positive intermediate progenitor cells was decreased in the cortical region, and it was found that the superficial layer is thinner and the generation of superficial layer neurons was decelerated and prolonged.
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