2013 Fiscal Year Final Research Report
Molecular disection of melanin-concenting hormone receptor 1
| Project/Area Number |
23500449
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Hiroshima University |
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Principal Investigator |
SAITO Yumiko 広島大学, 総合科学研究科, 教授 (00215568)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Gタンパク質共役型受容体 / 摂食 / シグナル伝達 / うつ不安 |
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| Research Abstract |
Melanin-concentrating hormone receptor 1 (MCHR1) is a G protein-coupled receptor (GPCR) that is highly expressed in the central nervous system. Many studies of genetically engineered animals and selective antagonists showed an important role of MCHR1 in the regulation of many physiological functions including energy homeostasis and emotional processing. By extensive biological and pharmacological studies of rat MCHR1, we have accomplished that (1) generation of 3D-modeling of gain-of-function mutant with G protein, (2) identification of two mutants that are sensitive for Gi but not for Gq activation, (3) generation of transgenic mice that selectively over express one of MCHR1-binding molecule in the CNS, and (4) molecular cloning and signaling pathway for four MCHR from frog. We further characterized ciliary targeting sequence of MCHR1. These results will provide important information to better understand the complex mechanism for MCHR1 activation in vivo.
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