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2013 Fiscal Year Final Research Report

Physiological role of neuronal glutamate transporters: control of extrasynaptic glutamate diffusion-mediated modification of synaptic transmission

Research Project

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Project/Area Number 23500457
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

SATAKE Shin'Ichiro  生理学研究所, 生体情報研究系, 助教 (30360340)

Project Period (FY) 2011 – 2013
Keywordsエタノール / グルタミン酸輸送体 / 小脳 / プルキンエ細胞 / 登上線維 / 籠細胞
Research Abstract

In response to repetitive activation of the climbing fibers (CFs), GABA release at basket cell (BC)-Purkinje cell (PC) synapses in the cerebellar cortex is suppressed through extrasynaptic diffusion of the CF transmitter glutamate and following activation of AMPA receptors that expressed on BC axon terminals. We found that the CF-induced presynaptic inhibition of cerebellar GABAergic transmission is suppressed by ethanol (EtOH) at a clinically relevant concentration (25-50 mM). Pharmacological blockade of the neuronal glutamate transporter EAAT4 not only augmented the CF-induced inhibition of GABAergic transmission but also abolished the suppressive action of EtOH on the CF-induced inhibition. This EtOH action was diminished by inhibitors for protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K). These results suggest that EtOH acutely potentiates EAAT4-mediated glutamate uptake in PCs through PKC/PI3K-mediated protein phosphorylation.

  • Research Products

    (11 results)

All 2014 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Book (1 results)

  • [Journal Article] Enhanced inhibi- tory neurotransmission in the cerebel- lar cortex of Atp1a3 -deficient hetero- zygous mice2013

    • Author(s)
      Keiko Ikeda, Shin'Ichiro Satake, Tatsushi Onaka, Hiroki Sugimoto, Naoki Takeda, Keiji Imoto, Kiyoshi Kawakami
    • Journal Title

      Journal of Physiology

      Volume: 591 Pages: 3433-3449

    • DOI

      10.1113/jphysiol.2012.247817

    • Peer Reviewed
  • [Journal Article] Paired-pulse facil- itation of multivesicular release and intersynaptic spillover of glutamate at rat cerebellar granule cell-inter- neurone synapses2012

    • Author(s)
      Shin'Ichiro Satake, Tsuyoshi Inoue, Keiji Imoto
    • Journal Title

      Journal of Physi- ology

      Volume: 590 Pages: 5653-5675

    • DOI

      10.1113/jphysiol.2012.234070

    • Peer Reviewed
  • [Journal Article] ニューロン型グルタミン酸トランスポーターが制御する異種シナプス間クロストーク2011

    • Author(s)
      佐竹 伸一郎, 小西 史朗, 井本 敬二
    • Journal Title

      脳 21

      Volume: 14 Pages: 320-325

  • [Presentation] 3 次元拡散モデルを用いた Ca^<2+> チャネル‐開口放出センサー間距離の推測2014

    • Author(s)
      井本 敬二, 佐竹 伸一郎
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      20140316-18
  • [Presentation] Increased inhibi- tory neurotransmission in the cerebel- lum of the Atp1a3 -deficient hetero- zygous mice2013

    • Author(s)
      Keiko Ikeda, Shin'Ichiro Satake, Kiyoshi Kawakami
    • Organizer
      Second Symposium on ATP1A3 in Disease : Genotype/Pheno- type Correlations, Modelling and Identification of Potential Targets for Treatment
    • Place of Presentation
      Catholic University School of Medicine(Rome, Italy)
    • Year and Date
      20130923-24
  • [Presentation] Atp1a3 +/- マウス小脳皮質において登上線維伝達物質のシナプス外拡散は強く抑制されている2013

    • Author(s)
      佐竹 伸一郎, 池田 啓子, 川上 潔, 井本 敬二
    • Organizer
      Neuro2013 (第36回日本神経科学大会・第56回日本神経化学会大会・第23回日本神経回路学会大会合同大会)
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      20130620-23
  • [Presentation] 小脳顆粒細胞のシナプス小胞多重性放出におけるCa v 2.1 チャネルの役割2012

    • Author(s)
      佐竹 伸一郎, 井本 敬二
    • Organizer
      Neuroscience 2012(第35回日本神経科学大会)
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      20120918-21
  • [Presentation] DYT12モデルマウスの小脳抑制系神経伝達の変化2012

    • Author(s)
      池田 啓子, 佐竹 伸一郎, 尾仲 達史, 竹田 直樹, 井本 敬二, 川上 潔
    • Organizer
      Neuroscience 2012(第35回日本神経科学大会)
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      20120918-21
  • [Presentation] ラット小脳皮質において G_<i/o> 共役型受容体が仲介するシナプス前抑制における多様性2011

    • Author(s)
      佐竹 伸一郎, 井本 敬二
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      大雪アリーナ(旭川市)
    • Year and Date
      20110921-23
  • [Presentation] 小脳顆粒細胞‐分子層介在ニューロン間興奮性伝達のシナプス前性増強における多様性2011

    • Author(s)
      佐竹 伸一郎, 井本 敬二
    • Organizer
      Neuroscience 2011(第34回日本神経科学大会)
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      20110914-17
  • [Book] Physiological interactions between neurons and glia : roles of transporters in the control of intersynaptic crosstalk. In Glial Cells: Embryonic Development, Types/Functions and Role in Disease. (Eds. Charanjit Kaur and Eng-Ang Ling)2013

    • Author(s)
      Shiro Konishi, Shin'Ichiro Satake
    • Total Pages
      177-192
    • Publisher
      New York : Nova Science Publishers

URL: 

Published: 2015-07-16  

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