2013 Fiscal Year Final Research Report
Development of ratiometric molecular probes for monitoring oxygen levels in living cells
| Project/Area Number |
23500511
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | りん光 / レシオ / 蛍光 / 酸素 / イリジウム錯体 / 細胞 |
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| Research Abstract |
Ratiometric molecular probes for the measurement of oxygen levels in lipid membranes and living cells were designed and synthesized. The synthesized molecules showed an oxygen-insensitive fluorescence with coumarin 343 and an oxygen-sensitive phosphorescence with iridium complexes. The ratio of the phosphorescence intensity for the fluorescence intensity increased with the decrease of oxygen concentration in the solution under the presence of lipid membranes. These probes were added to the cell medium and cells were incubated. The fluorescence and phosphorescence images of living cells were obtained by using microscopic techniques. These results exhibited that the ratiometric molecular probe has a high advantage for the measurements and the images of oxygen levels in living cells.
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[Journal Article] Tetraalkoxyphneanthrene-Fused Dehydroannulenes: Synthisis, Self-Assembly, and Electronic, Optical and Electrochemical Properties2013
Author(s)
S. Kato, N. Takahashi, H. Tanaka, A. Kobayashi, T. Yoshihara, S. Tobita, T. Yamanobe, H. Uehara, Y. Nakamura
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Journal Title
Chem. Eur. J
Volume: 19
Pages: 12138-12151
DOI
Peer Reviewed
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