2013 Fiscal Year Final Research Report
Oxygen imaging by quantum dots for detection of intra-tumor hypoxia
| Project/Area Number |
23500520
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Saga University |
|---|
Principal Investigator |
TAKAHASHI Eiji 佐賀大学, 工学(系)研究科(研究院), 教授 (30206792)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
JIN Takashi 独立行政法人理化学研究所 (80206367)
|
|---|
| Research Collaborator |
HIGASHI Kenji 佐賀大学, 大学院生
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 量子ドット / 酸素 / 培養細胞 / イメージング |
|---|
| Research Abstract |
Hypoxic cells in solid tumor define the malignancy. Thus, imaging of hypoxic cells in solid tumor is of significant importance. Here, we attempted to use nanometer-scale semiconductor particles called quantum dots (QDs) as an optical probe for hypoxic cells. Fluorescence intensity of the newly synthesized L-cysteine capped QD, loaded into cultured cells, changed according to the intracellular oxygen concentration. However, the fluorescence intensity was lower than expected from the in vitro study. We also found significant photo bleaching of the QD fluorescence. These appear to hinder the immediate use of the QD as a nanoscale optical oxygen probe.
|
