2013 Fiscal Year Final Research Report

Controlled release of growth factors for bone regeneration

Research Project

Project/Area Number	23500543
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Biomedical engineering/Biological material science
Research Institution	Osaka City University
Principal Investigator	TACHIBANA Akira 大阪市立大学, 大学院工学研究科, 准教授 (80305614)
Co-Investigator(Kenkyū-buntansha)	TANABE Toshizumi 大阪市立大学, 大学院・工学研究科, 教授 (20315972)
Project Period (FY)	2011 – 2013
Keywords	徐放システム / FGF2 / キチン
Research Abstract	Chitin gel was obtained by the acetylation of chitosan in solution. Basic growth factors did not bind to non treated chitin gel, whereas tightly bound to carboxymethylated chitin gel. Chitin binding domain-FGF-2 was bound to chitin gel and not released by PBS washing. Chitin binding domain-FGF-2 was released by degradation of chitin gel with lysozyme. During degradation of chitin gel with lysozyme, chitin fragment was released at a burst, but chitin binding domain-FGF-2 was not. Most of chitin binding domain-FGF-2 that was released from gel were re-bound to the gel, only low amount of FGF-2 was released out. Therefore, ideal controlled release without a burst release was realized.

(2 results)