Development of the new sonodynamic cancer therapy combined with nano or micro technology

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500569
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Yokohama College of Pharmacy
• Principal Investigator: IWASE Yumiko 横浜薬科大学, 薬学部, 講師 (00521882)
• Co-Investigator(Kenkyū-buntansha): UMEMURA Shin-ichirou 東北大学, 工学部, 教授 (20402787) ; YUMITA Nagahiko 横浜薬科大学, 薬学部, 教授 (40191481)
• Project Period (FY): 2011 – 2013
• Keywords: アポトーシス / ドラッグデリバリーシステム / ナノ粒子 / マイクロ微粒子 / 超音波

Research Abstract

In this study, we investigated the sonodynamically induced antitumor effect of functionalized fullerenes. The antitumor effects of functionalized fullerenes in combination with ultrasound at 2 MHz were screened. The ultrasonically induced damage on isolated sarcoma 180 cells was enhanced significantly with polyhydroxy fullerenes (PHF) or pyrrolidine tris-acid fullerenes (PTF). In the experimental treatment, the combined with ultrasonic exposure and PHF inhibited the growth of the inoculated colon 26 tumors. The significant reduction by histidine of ultrasonically induced cell damage enhanced by PHF suggests that the enhancement was due to sonodynamic generation of active oxygen such as singlet oxygen by functionalized fullerenes. The involvement of singlet oxygen was also confirmed by ESR spectometory. These results support the possibility of clinical application of sonodynamic treatment using water soluble functionalized fullerene.

Research Products

• [Journal Article] Sonodynamically Induced Anticancer Effects by Water-soluble Fullerene Derivative (2014)
  • Author(s): Iwase Y, Yumita N, Imaizumi T, Sakurazawa A, Kaya Y, Nishi K, Ikeda T, Umemura S, Chen F, Momose Y
  • Journal Title: Drug Metabolism Reviews Volume: 45 Pages: 98-98
• [Journal Article] Sonodynamically Induced Anticancer Effects by Functionalized fullerenes (2013)
  • Author(s): Yumita N, Iwase Y, Imaizumi T, Sakurazawa A, Kaya Y, Nishi K, Ikeda T, Umemura S, Chen F, Momose Y
  • Journal Title: Anticancer Res Volume: 33 Pages: 700-6
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by Novel Porphyrin Derivative (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Komatsu H, Fukai T, Ikeda T, Umemura S, Momose Y
  • Journal Title: Theranostics Volume: 2 Pages: 880-888
  • Peer Reviewed
• [Journal Article] Apoptosis of HL-60 Cells Induced by Sonodynamic Therapy with Zinc Phthalocyanine Tetrasulfonate (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda K, Onodera K, Fukai T, Umemura S, Momose Y
  • Journal Title: Pharmacometrics Volume: 82 Pages: 75-83
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deut erio-Porphynyl (IX)-6-7-diaspartic acid (ATX-S10) (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda T, Fukai T, Onodera K, Takeda K, Umemura S, Okudaira K, Momose Y
  • Journal Title: Biochemistry & Analytical Biochemistry Pages: 2-7
  • Peer Reviewed
• [Journal Article] Sonodynamically-induced antitumor effect of mono-l-aspartyl chlorin e6 (NPe6) (2011)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda T, Komatsu H, Fukai T, Onodera K, Nishi H, Takeda K, Umemura S, Okudaira K, Momose Y
  • Journal Title: Anticancer Res Volume: 31 Pages: 501-6
  • Peer Reviewed
• [Journal Article] Sonodynamic Antitumor Effect of Zinc Phthalocyanine Tetrasulfate on Murine Solid Tumor colon 26 (2011)
  • Author(s): Yumita N, Iwase Y, Onodera K, Nishi H, Takeda K, Fukai, T, Umemura S, Okudaira K, Momose Y
  • Journal Title: Pharmacometrics Volume: 80 Pages: 13-20
  • Peer Reviewed
• [Presentation] Involvement of Reactive Oxygen Species in Apoptosis Induced by Sonodynamic Therapy with Chloroaluminum Phthalocyanine Tetrasulfonate (2013)
  • Author(s): Nagahiko Yumita, Saeko Nakamura, Yumiko Iwase, Toshio Fukai, Toshihiko Ikeda, Shin-ichro Umemura
  • Organizer: SFRBM's 20th Annual Meeting
  • Place of Presentation: サンアントニオ
  • Year and Date: 20131119-26
• [Presentation] Sonodynamically Induced Anticancer Effects by Functionalized fullerenes (2013)
  • Author(s): Nagahiko Yumita, Yumiko Iwase, Takahiro Imaizumi, Ai Sakurazawa, Yuka Kaya, Koji Nishi, Shin-ichiro Umemura, Fu-Shin Chen, Yasunori Momose, Yoshihiko Ikeda
  • Organizer: 10th International ISSX Meeting
  • Place of Presentation: トロント
  • Year and Date: 20130929-1003
• [Presentation] Sonodynamically Induced Antitumor Effect of Novel Porphyrin Derivative (2013)
  • Author(s): Nagahiko Yumita, Yumiko Iwase, Koji Nishi, Toshihiko Ikeda, Toshio Fukai, Shin-ichiro Umemura
  • Organizer: 第73回世界薬学大会(FIP2013)
  • Place of Presentation: ダブリン
  • Year and Date: 20130831-0905
• [Presentation] ATX-S10 の音響化学的活性化によるアポトーシス誘導 (2012)
  • Author(s): 弓田長彦, 岩瀬由未子, 深井俊夫, 武田収功, 小野寺憲治, 池田敏彦, 梅村晋一郎, 奥平和穂, 百瀬弥寿徳
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] Sonodynamically induced apoptosis by ATX-S10 in HL-60 cells (2011)
  • Author(s): Yumiko Iwase, Kazuho Okudaira, Shin-ichiro Umemura, Nagahiko Yumita
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04