Study on selective cancer therapy using apoptosis induced by sonodynamically activated drug

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500570
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Yokohama College of Pharmacy
• Principal Investigator: FUKAI Toshio 横浜薬科大学, 薬学部, 教授 (10057755)
• Co-Investigator(Kenkyū-buntansha): YUMITA Nagahiko 横浜薬科大学, 薬学部, 教授 (40191481) ; IWASE Yumiko 横浜薬科大学, 薬学部, 講師 (00521882)
• Project Period (FY): 2011 – 2013
• Keywords: アポトーシス / 一重項酸素 / 活性酸素種 / 超音波 / 音響化学療法

Research Abstract

Recently, we have been studying antitumor activation of porphyrin compounds by ultrasound. The present study aims to the application of sonodynamically inducted apoptosis by porphyrin compounds for cancer treatment. Cells treated with ultrasound and porphyrin sompounds such as Porfimer Sodium, ATX-70, ATX-S10, NPe6 and DCPH-Na clearly showed membrane blebbing and cell shrinkag. Also, DNA ladder formation and caspase-3 activation were pbserved in cells treated with ultrasound and porphyrin compounds but not in order treatments. In addition, the combination of porphyin compounds and ultrasound enhanced nitroxide generation measured by ESR. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. These findings suggest that ultrasonically generated reactive oxygen species such as singlet oxygen is an important mediator of sonodynamically induced apoptosis.

Research Products

• [Journal Article] Sonodynamically Induced Anticancer Effects by Water-soluble Fullerene Derivative (2014)
  • Author(s): Iwase Y, Yumita N, Imaizumi T, Sakurazawa A, Kaya Y, Nishi K, Ikeda T, Umemura S, Chen F, Momose Y
  • Journal Title: Drug Metabolism Reviews Volume: 45 Pages: 98-98
• [Journal Article] Sonodynamically Induced Anticancer Effects by Functionalized fullerenes (2013)
  • Author(s): Yumita N, Iwase Y, Imaizumi T, Sakurazawa A, Kaya Y, Nishi K, Ikeda T, Umemura S, Chen F, Momose Y
  • Journal Title: Anticancer Res Volume: 33 Pages: 700-6
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by Novel Porphyrin Derivative (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Komatsu H, Fukai T, Ikeda T, Umemura S, Momose Y
  • Journal Title: Theranostics Volume: 2 Pages: 880-888
  • Peer Reviewed
• [Journal Article] Apoptosis of HL-60 Cells Induced by Sonodynamic Therapy with Zinc Phthalocyanine Tetrasulfonate (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda K, Onodera K, Fukai T, Umemura S, Momose Y
  • Journal Title: Pharmacometrics Volume: 82 Pages: 75-83
  • Peer Reviewed
• [Journal Article] Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis by 4-formyloximeetylidene-3-hydroxyl-2-vinyl-deut erio-Porphynyl (IX)-6-7-diaspartic acid (ATX-S10) (2012)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda T, Fukai T, Onodera K, Takeda K, Umemura S, Okudaira K, Momose Y
  • Journal Title: Biochemistry & Analytical Biochemistry
  • Peer Reviewed
• [Journal Article] Sonodynamically-induced antitumor effect of mono-l-aspartyl chlorin e6 (NPe6) (2011)
  • Author(s): Yumita N, Iwase Y, Nishi K, Ikeda T, Komatsu H, Fukai T, Onodera K, Nishi H, Takeda K, Umemura S, Okudaira K, Momose Y
  • Journal Title: Anticancer Res Volume: 31 Pages: 501-6
  • Peer Reviewed
• [Journal Article] Sonodynamic Antitumor Effect of Zinc Phthalocyanine Tetrasulfate on Murine Solid Tumor colon 26 (2011)
  • Author(s): Yumita N, Iwase Y, Onodera K, Nishi H, Takeda K, Fukai, T, Umemura S, Okudaira K, Momose Y
  • Journal Title: Pharmacometrics Volume: 80 Pages: 13-20
  • Peer Reviewed
• [Presentation] Involvement of Reactive Oxygen Species in Apoptosis Induced by Sonodynamic Therapy with Chloroaluminum Phthalocyanine Tetrasulfonate (2013)
  • Author(s): Nagahiko Yumita, Saeko Nakamura, Yumiko Iwase, Toshio Fukai, Toshihiko Ikeda, Shin-ichro Umemura
  • Organizer: SFRBM's 20th Annual Meeting
  • Place of Presentation: サンアントニオ
  • Year and Date: 20131119-26
• [Presentation] Sonodynamically Induced Anticancer Effects by Functionalized fullerenes (2013)
  • Author(s): Nagahiko Yumita, Yumiko Iwase, Takahiro Imaizumi, Ai Sakurazawa, Yuka Kaya, Koji Nishi, Shin-ichiro Umemura, Fu-Shin Chen, Yasunori Momose, Yoshihiko Ikeda
  • Organizer: 10th International ISSX Meeting
  • Place of Presentation: トロント
  • Year and Date: 20130929-1003
• [Presentation] Sonodynamically Induced Antitumor Effect of Novel Porphyrin Derivative (2013)
  • Author(s): Nagahiko Yumita, Yumiko Iwase, Koji Nishi, Toshihiko Ikeda, Toshio Fukai, Shin-ichiro Umemura
  • Organizer: 第73回世界薬学大会(FIP2013)
  • Place of Presentation: ダブリン
  • Year and Date: 20130831-0905
• [Presentation] ATX-S10の音響化学的活性化によるアポトーシス誘導 (2012)
  • Author(s): 弓田長彦, 岩瀬由未子, 深井俊夫, 武田収功, 小野寺憲治, 池田敏彦, 梅村晋一郎, 奥平和穂, 百瀬弥寿徳
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] Sonodynamically induced apoptosis by ATX-S10 in HL-60 cells (2011)
  • Author(s): Yumiko Iwase, Kazuho Okudaira, Shin-ichiro Umemura, Nagahiko Yumita
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04