Function and structure of food-derived and endogenous collagen peptide in damaged regions of skin

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University
• Principal Investigator: SATO KENJI 京都府立大学, 生命環境科学研究科(系), 教授 (00202094)
• Co-Investigator(Kenkyū-buntansha): SHIGEMURA Yasutaka 東京家政大学, 短期大学部, 講師 (20373178)
• Project Period (FY): 2011 – 2013
• Keywords: コラーゲン / ペプチド / 線維芽細胞 / 創傷治癒 / 皮膚 / Pro-Hyp

Research Abstract

The present study demonstrates that occurrence of Pro-Hyp in the inflammatory and wound healing regions of mice. Pro-Hyp has been demonstrated to be present human blood after ingestion of collagen peptide. In addition, primary cultured fibroblast from mouse skin expressed mesenchymal stem cell marker (p75NTR) and peptide transporters and incorporated Pro-Hyp and started to grow on collagen gel, which were extensively decreased after passage. The present study demonstrates the significant role of Pro-Hyp in wound healing process and explores mechanism for enhance meant of wound healing by ingestion of collagen peptide.

Research Products

• [Journal Article] Dose-dependent changes in the levels of free and peptide forms of hydroxyproline in human plasma after collagen hydrolysate ingestion (2014)
  • Author(s): Shigemura, Y., Kubomura, D., Sato, Y., Sato, K
  • Journal Title: Food Chemistry Volume: 159 Pages: 328-332
  • Peer Reviewed
• [Journal Article] Identification of food-derived elastin peptide, prolyl-glycine (Pro-Gly), in human blood after ingestion of elastin hydrolysate (2012)
  • Author(s): Shigemura, Y., Nakaba, M., Shiratsuchi, E., Suyama, M., Yamada, M., Kiyono, T. Fukamizu, K., Park, E. Y., Nakamura, Y., Sato, K
  • Journal Title: J. Agric. Food Chem Volume: 60 Pages: 5128-5133
  • Peer Reviewed
• [Journal Article] Identification of a novel food-derived collagen peptide, hydroxyprolyl-glycine, in human peripheral blood by pre-column derivatisation with phenyl isothiocyanate (2011)
  • Author(s): Shigemura, Y., Akaba, S., Kawashima, E., Park E.-Y. Nakamura, Y., Sato, K
  • Journal Title: Food Chemistry Volume: 129 Pages: 1019-1024
  • Peer Reviewed
• [Journal Article] 美容とアンチエイジングのための素材~コラーゲンペプチドを中心に~ (2011)
  • Author(s): 佐藤健司
  • Journal Title: Food Style 21 Volume: 15 Pages: 34-37
• [Presentation] Pro-Hyp の皮膚線維芽細胞増殖促進効果 (2014)
  • Author(s): 佐藤 健司
  • Organizer: 第14回抗加齢医学総会
  • Place of Presentation: 大阪
  • Year and Date: 20140606-08
• [Presentation] Incorporation of food-derived collagen peptides, Pro-Hyp and Hyp-Gly, into primary cultured fibroblast from mice skin - possible trigger for growth of fibroblast (2013)
  • Author(s): Satou, K
  • Organizer: ISNFF Annual Conference
  • Place of Presentation: Taipei
  • Year and Date: 20131105-09
• [Presentation] ペプチドの機能 (2013)
  • Author(s): 佐藤 健司
  • Organizer: 日本臨床栄養学会・日本臨床栄養協会 第11回連合大会
  • Place of Presentation: 京都
  • Year and Date: 20131004-06
• [Presentation] 食事由来コラーゲンペプチドの真皮に及ぼす影響 (2013)
  • Author(s): 佐藤 健司
  • Organizer: 第13回抗加齢医学総会
  • Place of Presentation: 横浜
  • Year and Date: 20130628-30
• [Presentation] 真皮コラーケンの産生メカニスム (2012)
  • Author(s): 佐藤 健司
  • Organizer: 第12回抗加齢医学総会
  • Place of Presentation: 横浜
  • Year and Date: 20120622-25
• [Presentation] Food-derived collagen peptides in human blood-structure and biological functions (2011)
  • Author(s): Sato, K
  • Organizer: ICOFF 2011
  • Place of Presentation: Taipei, Chinese Taipei
  • Year and Date: 20111120-23
• [Presentation] Impact of extracellular matrix protein hydrolysates on human health (2011)
  • Author(s): Sato, K
  • Organizer: matrix protein hydrolysates on human health
  • Place of Presentation: Cincinnati, USA
  • Year and Date: 20110501-04
• [Book] Fish-elastin hydrolysate. In "Marine Proteins and Peptides" Kim, S.-K. Edit (2013)
  • Author(s): Shiratsuchi, E., Nakaba, M., Shigemura, Y., Yamada, M., Sato, K
  • Total Pages: 467-486
  • Publisher: Wiley-Blackwell
• [Book] Effect of marine collagen peptide on skin condition. In "Marine Cosmeceuticals Trends and Prospective" Kim, S.-K. Edit (2012)
  • Author(s): Sato, K., Shigemura, Y
  • Total Pages: 125-132
  • Publisher: CRC Press, New York
• [Book] Proteins and peptides for complicated disease type. In "Bioactive Food Proteins and Peptides. Applicationa in Human Health" Hettirarachchy, N. S., Sato, K., Marshall, M. R., Kannan, A. Edit
  • Author(s): Sato, K
  • Total Pages: 237-253
  • Publisher: CRC Press. Taylor & Francis Group, Boca Raton, FL, USA
• [Book] ペプチドの吸収「食品機能性成分の吸収・代謝機構」 宮澤陽夫監修
  • Author(s): 佐藤健司, 依田悠里
  • Total Pages: 96-104
  • Publisher: シーエムシー出版