Investigation of RNA binding proteins that specifically suppress a miRNA maturation in tumor cells.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23501264
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Tohoku University
• Principal Investigator: YASUDA Jun 東北大学, 東北メディカル・メガバンク機構, 教授 (00281684)
• Project Period (FY): 2011 – 2013
• Keywords: マイクロRNA / 発がん / RNA結合タンパク質

Research Abstract

I have identified a RNA binding protein which specifically binds to a miRNA precursor in medulloblastoma cells. Suppression of the protein by the use of siRNAs causes the increase of the mature miRNA whose precursor binds to the target protein. I also test if the downstream target genes of the increased miRNA are suppressed by the siRNA treatment for the RNA binding protein. Finally, I analyzed the genomic sequences of the RNA binding proteins in the human tumor cells to test the relationship between the RNA binding proteins and tumorigenesis in human tumor cell lines.

Research Products

• [Presentation] Erb3-binding protein 1 (Ebp1) may suppress miR-124 maturation in the Medulloblastoma of the Ptc1 Heterozygous Mice (2012)
  • Author(s): J.Yasuda, K.Yaginuma, Y.Saiki, Y.Sugitani, C.Obuse, T.Noda
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-21
• [Presentation] The cloning and characterization of specific modifiers of miRNA maturation in the medulloblastoma of the ptc1 heterozygous mice (2011)
  • Author(s): J.Yasuda, K.Yaginuma, Y.Saiki, Y.Sugitani, C.Obuse, T.Noda
  • Organizer: CELL Symposia for Regulatory RNAs 2011
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-11