Single-cell analysis of T-cell receptor repertoire of HTLV-1 Tax-specific cytotoxic T cells in patients with adult T-cell leukemia/lymphoma.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23501286
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor immunology
• Research Institution: Jichi Medical University
• Principal Investigator: KANDA Yoshinobu 自治医科大学, 医学部, 教授 (30334379)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Rie 自治医科大学, 医学部, 助教 (80365262)
• Project Period (FY): 2011 – 2013
• Keywords: 細胞傷害性T細胞 / レパトア / 成人T細胞性白血病 / HTLV-I / 造血幹細胞移植

Research Abstract

We analyzed the T-cell receptor repertoire of HTLV-1 Tax-specific cytotoxic T cells in patients with adult T-cell leukemia/lymphoma. We found that the Tax-CTLs share P-D/P-R motif, persist as effector memory CTLs for long term after transplantation, and show universal killing activity against third-party HTLV-I infected cells. We are planning a redirected T-cell immunotherapy using T-cell receptor alpha/beta genes from these clones.

Research Products

• [Journal Article] Universal cytotoxic activity of a HTLV-1 Tax-specific T cell clone from an HLA-A*24 : 02+ patient with adult T-cell leukemia against a variety of HTLV-I-infected T-cells (2014)
  • Author(s): Yukie Tanaka, Rie Yamazaki, Kiriko Terasako-Saito, Hideki Nakasone, Yu Akahoshi, Hiroshi Nakano, Tomotaka Ugai, Hidenori Wada, Ryoko Yamasaki, Yuko Ishihara, Koji Kawamura, Kana Sakamoto, Masahiro Ashizawa, Miki Sato, Shun-ichi Kimura, Misato Kikuchi, Shinichi Kako, Junya Kanda, Aki Tanihara, Junji Nishida, Yoshinobu Kanda
  • Journal Title: Immunology Letters Volume: 158 Pages: 120-125
  • DOI: 10.1016/j.imlet.2013.12.016
• [Journal Article] Single-cell analysis of T-cell receptor-β repertoire of HLA-A*2402-restricted cytomegalovirus pp65-specific cytotoxic T-cells in donor-patient pairs undergoing allogeneic hematopoietic stem cell transplantation (2014)
  • Author(s): Hideki Nakasone, Yukie Tanaka, Rie Yamazaki, Miki Sato, Kiriko Terasako, Kana Sakamoto, Ryoko Yamasaki, Hidenori Wada, Yuko Ishihara, Koji Kawamura, Tomohito Machishima, Masahiro Ashizawa, Shun-ichi Kimura, Misato Kikuchi, Aki Tanihara, Junya Kanda, Shinichi Kako, Junji Nishida, Yoshinobu Kanda
  • Journal Title: Bone Marrow Transplantation Volume: 49 Pages: 87-94
  • DOI: 10.1038/bmt.2013.122
• [Journal Article] Allotype analysis to distinguish the origin of varicella-zoster virus immunoglobulin G after allogeneic stem cell transplantation (2013)
  • Author(s): Rie Yamazaki, Hideki Nakasone, Yukie Tanaka, Miki Sato, Kiriko Terasako, Hidenori Wada, Yuko Ishihara, Koji Kawamura, Kana Sakamoto, Masahiro Ashizawa, Tomohito Machishima, Shun-ichi Kimura, Misato Kikuchi, Shinya Okuda, Shinichi Kako, Junya Kanda, Aki Tanihara, Junji Nishida, Yoshinobu Kanda
  • Journal Title: Biology of Blood and Marrow Transplantation Volume: 19 Pages: 1013-1020
  • DOI: 10.1016/j.bbmt.2013.04.007
• [Journal Article] Long-term persistence of limited HTLV-I Tax-specific cytotoxic T cell clones in a patient with adult T cell leukemia/lymphoma after allogeneic stem cell transplantation (2012)
  • Author(s): Yukie Tanaka, Hideki Nakasone, Rie Yamazaki, Hidenori Wada, Yuko Ishihara, Koji Kawamura, Kana Sakamoto, Masahiro Ashizawa, Tomohito Machishima, Miki Sato, Kiriko Terasako, Shun-ichi Kimura, Misato Kikuchi, Shinya Okuda, Shinichi Kako, Junya Kanda, Aki Tanihara, Junji Nishida, Yoshinobu Kanda
  • Journal Title: Journal of Clinical Immunology Volume: 32 Pages: 1340-1352
  • DOI: 10.1007/s10875-012-9729-5
• [Journal Article] Impact of high-/middle-molecular-weight adiponectin on the synthesis and regulation of extracellular matrix in dermal fibroblasts
  • Author(s): Hideki Nakasone, Kiriko Terasako-Saito, Rie Yamazaki, Miki Sato, Yukie Tanaka, Kana Sakamoto, Masakazu Kurita,, Ryoko Yamasaki, Hidenori Wada, Yuko Ishihara, Koji Kawamura, Tomohito Machishima, Masahiro Ashizawa, Shun-ichi Kimura, Misato Kikuchi, Aki Tanihara, Junya Kanda, Shinichi Kako, Junji Nishida, Shigeki Yamada and Yoshinobu Kanda
  • Journal Title: Experimental Hematology Volume: (in press)
  • DOI: 10.1016/j.exphem.2013.12.009