Improved immunogenicity of fusions between ethanol-treated cancer cells and dendritic cells exposed to dual TLR stimulation

2014 Fiscal Year Final Research Report

• Project/Area Number: 23501289
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor immunology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: KOIDO Shigeo 東京慈恵会医科大学, 医学部, 准教授 (70266617)
• Co-Investigator(Kenkyū-buntansha): HOMMA Sadamu 東京慈恵会医科大学, 医学部, 教授 (50192323)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 樹状細胞 / がん細胞 / がん免疫 / 細胞障害性Ｔ細胞 / がんワクチン / 融合細胞

Outline of Final Research Achievements

Fusions of ethanol-treated tumor cells and dual Toll-like receptors (TLRs)-stimulated dendritic cells (DCs) (E-tumor/FCs) inhibited the production of multiple immunesuppressive soluble factors including TGF-β1 and up-regulated the production of IL-12p70 and HSP90α. Importantly, E-tumor/FCs activated T cells capable of producing high levels of IFN-γ, resulting in augmented MUC1-specific cytotoxic T lymphocyte (CTL) induction. Collectively, our results illustrate the synergy between ethanol-treated whole tumor cells and dual TLRs-stimulated DCs in inducing augmented CTL responses in vitro by fusion cell preparations. The alternative system is simple and may provide a platform for adoptive immunotherapy.

Free Research Field

がん免疫