Analysis of the new gene in connection with permeation of the lung adenocarcinoma in the genome PCR method and the FISH method

2013 Fiscal Year Final Research Report

• Project/Area Number: 23501294
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: University of Tsukuba
• Principal Investigator: MINAMI YUKO 筑波大学, 医学医療系, 准教授 (20466676)
• Co-Investigator(Kenkyū-buntansha): NOGUCHI Masayuki 筑波大学, 医学医療系, 教授 (00198582)
• Project Period (FY): 2011 – 2013
• Keywords: 小型肺腺癌 / 3q26 / ECT2 / FISH / qPCR / 免疫染色 / 予後関連遺伝子

Research Abstract

Genetic abnormality in early-stage lung adenocarcinoma was examined to search for new prognostic biomarkers. In situ lung adenocarcinomas and small but invasive adenocarcinomas were examined by array-comparative genomic hybridization (array-CGH), and candidate genes of interest were screened. To examine gene abnormalities, various types of lung carcinoma were examined by quantitative real-time genomic PCR (qPCR) and immunohistochemistry (IHC). Array-CGH demonstrated frequent amplification at chromosome 3q26, and we focused on the epithelial cell transforming sequence 2 (ECT2) oncogene. Overexpression of ECT2 in invasive adenocarcinoma was associated with disease-free survival and overall survival of patients with lung adenocarcinoma. These results were verified using another set of early-stage adenocarcinomas resected at another hospital. Abnormality of the ECT2 gene would be applicable as a new biomarker for prognostication of patients with lung adenocarcinoma.

Research Products

• [Journal Article] ECT2 overexpression as a new prognostic biomarker for early-stage lung adenocarcinoma (2014)
  • Author(s): Murata Y, Minami Y, Iwakawa R, Yokota J, Usui S, Tsuta K, Shiraishi K, Sakashita S, Satomi K, Iijima T, Noguchi M
  • Journal Title: Cancer Sci Volume: 105(4) Pages: 490-497
  • DOI: 10.1111/cas.12363
  • Peer Reviewed
• [Journal Article] ECT2 serves as a new biomarker for early-stage lung adenocarcinoma. A FISH image for a tumor showing high ECT2 amplification. Red signal shows the ECT2 gene, and green signal shows chromosome 3 enumeration (2014)
  • Journal Title: Cancer Sci Volume: 105(4) Pages: April cover
  • DOI: 10.1111/cas.12384
  • Peer Reviewed
• [Presentation] ECT2 発現亢進は肺腺癌の新規予後予測マーカーである (2013)
  • Author(s): 村田佳彦、南優子、岩川麗香、横田淳、野口雅之
  • Organizer: 第54回日本肺癌学会総会
  • Place of Presentation: ホテルニューオータニ東京
  • Year and Date: 20131121-22
• [Presentation] ECT2 overexpression and gene alteration are associated with the outcome of early-stage lung adenocarcinoma (2013)
  • Author(s): Minami Y, Murata Y, Iwakawa R, Yokota J, Noguchi M
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: Australia
  • Year and Date: 20131027-30
• [Presentation] 肺癌における3q26 領域の遺伝子増幅の意義 (2013)
  • Author(s): 村田佳彦、南優子、野口雅之
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: ホテルロイトン札幌
  • Year and Date: 20130606-08
• [Presentation] 肺癌における3q26 領域の遺伝子増幅 (2012)
  • Author(s): 村田佳彦、南優子、薄井真悟、野口雅之
  • Organizer: 第53回日本肺癌学会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 20121108-09
• [Presentation] 肺癌における3q26 領域の遺伝子増幅(Genomic amplification at 3q26 region in lung carcinoma) (2012)
  • Author(s): 村田佳彦、南優子、野口雅之
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: ホテルロイトン札幌
  • Year and Date: 20120919-21
• [Presentation] 小型肺腺癌のArray CGH 解析 (2011)
  • Author(s): 南優子、村田佳彦、野口雅之
  • Organizer: 第52回日本肺癌学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20111103-04
• [Presentation] Array CGH analysis of small-sized adenocarcinoma of the lung (2011)
  • Author(s): Minami Y, Murata Y, Noguchi M
  • Organizer: 14^<th> World conference on lung
  • Place of Presentation: Amsterdam, The Netherlands
  • Year and Date: 20110703-07
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/diagpatho/