Development of an early diagnostic system of human pancreatic cancer using metabolite profiling

2013 Fiscal Year Final Research Report

• Project/Area Number: 23501296
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: Kobe University
• Principal Investigator: HATANO Naoya 神戸大学, 医学(系)研究科(研究院), 特命助教 (10332280)
• Project Period (FY): 2011 – 2013
• Keywords: メタボロミクス / プロテオミクス / がん

Research Abstract

Cancer is leading cause of death in Japan. So, it is important to find patients with suspected cancer and take them to the hospital earlier. To find the patients, a low-cost, easy-to-use, and high-sensitive cancer screening method is required. Therefore, we proposed metabolite profiling using mass spectrometry as a cancer screening method. We established a method for human serum metabolomics. The method was applied to pancreatic cancer patients and healthy controls. Several metabolites were changed statistically significantly and contributed to the grouping. In order to understand how the metabolic changes occur, we also established the comparison proteomics method, which targeted the important metabolic enzyme group.

Research Products

• [Journal Article] Identification of amphiphysin 1 as an endogenous substrate for CDKL5, a protein kinase associated with X-linked neurodevelopmental disorder (2013)
  • Author(s): Sekiguchi M, Katayama S, Hatano N, Shigeri Y, Sueyoshi N, Kameshita I
  • Journal Title: Arch. Biochem. Biophys Volume: 535(2) Pages: 257-267
  • DOI: 10.1016/j.abb.2013.04.012
  • Peer Reviewed
• [Journal Article] PKL01, an Ndr kinase homologue in plant, shows tyrosine kinase activity (2012)
  • Author(s): Katayama S, Sugiyama Y, Hatano N, Terachi T, Sueyoshi N, Kameshita I
  • Journal Title: J. Biochem Volume: 152(4) Pages: 347-353
  • DOI: 10.1093/jb/mvs075
  • Peer Reviewed
• [Journal Article] Nitric oxide prevents phosphorylation of neuronal nitric oxide synthase at serine 1412 by inhibiting the Akt/PKB and CaM-KII signaling pathways (2012)
  • Author(s): Song T, Hatano N, Sugimoto K, Horii M, Yamaguchi F, Tokuda M, Miyamoto Y, Kambe T, Watanabe Y
  • Journal Title: Int. J. Mol. Med Volume: 30(1) Pages: 15-20
  • DOI: 10.3892/ijmm.2012.971
  • Peer Reviewed
• [Journal Article] Tumor suppressor cell adhesion molecule 1 (CADM1) is cleaved by a disintegrin and metalloprotease 10 (ADAM10) and subsequently cleaved by γ-secretase complex (2012)
  • Author(s): Nagara Y, Hagiyama M, Hatano N, Futai E, Suo S, Takaoka Y, Murakami Y, Ito A, Ishiura S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 417(1) Pages: 462-467
  • DOI: 10.1016/j.bbrc.2011.11.140
  • Peer Reviewed
• [Journal Article] Diagnosis of gastroenterological diseases by metabolome analysis using gas chromatography-mass spectrometry (2012)
  • Author(s): Yoshida M, Hatano N, Nishiumi S, Irino Y, Izumi Y, Takenawa T, Azuma T
  • Journal Title: J. Gastroenterol Volume: 47(1) Pages: 9-20
  • DOI: 10.1007/s00535-011-0493-8.
  • Peer Reviewed
• [Journal Article] Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer (2012)
  • Author(s): Ikeda A, Nishiumi S, Shinohara M, Yoshie T, Hatano N, Okuno T, Bamba T, Fukusaki E, Takenawa T, Azuma T, Yoshida M
  • Journal Title: Biomed. Chromatogr Volume: 26(5) Pages: 548-558
  • DOI: 10.1002/bmc.1671
  • Peer Reviewed
• [Journal Article] Serum fatty acid profiling of colorectal cancer by gas chromatography/mass spectrometry (2011)
  • Author(s): Kondo Y, Nishiumi S, Shinohara M, Hatano N, Ikeda A, Yoshie T, Kobayashi T, Shiomi Y, Iruno Y, Takenawa T, Azuma T, Yoshida M
  • Journal Title: Biomark. Med Volume: 5(4) Pages: 451-460
  • DOI: 10.2217/bmm.11.41
  • Peer Reviewed
• [Journal Article] Generation of autonomous activity of Ca2^<+>/calmodulindependent protein kinase kinase β by autophosphorylation (2011)
  • Author(s): Tokumitsu H, Hatano N, Fujimoto T, Yurimoto S, Kobayashi R
  • Journal Title: Biochemistry Volume: 50(38) Pages: 8193-8201
  • DOI: 10.1021/bi201005g
  • Peer Reviewed
• [Journal Article] Identification of a novel CaMKK substrate (2011)
  • Author(s): Fujimoto T, Hatano N, Nozaki N, Yurimoto S, Kobayashi R, Tokumitsu H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 410(1) Pages: 45-51
  • DOI: 10.1016/j.bbrc.2011.05.102
  • Peer Reviewed
• [Journal Article] GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis (2011)
  • Author(s): Ooi M, Nishiumi S, Yoshie T, Shiomi Y, Kohashi M, Fukunaga K, Nakamura S, Matsumoto T, Hatano N, Shinohara M, Irino Y, Takenawa T, Azuma T, Yohida M
  • Journal Title: Inflamm. Res Volume: 60(9) Pages: 831-840
  • DOI: 10.1007/s00011-011-0340-7
  • Peer Reviewed
• [Presentation] 乳がん細胞でのPI3K インヒビターによる代謝変化 (2013)
  • Author(s): 波多野 直哉、入野 康宏、竹縄 忠臣
  • Organizer: 第86回日本生化学大会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2013-09-12
• [Presentation] Metabolome and proteome analysis of cancer (2013)
  • Author(s): 波多野 直哉
  • Organizer: SYMPOSIUM : A career in calcium signaling
  • Place of Presentation: 高松・香川大学医学部
  • Year and Date: 2013-03-29
  • Invited
• [Presentation] Characterization of breast cancer cells with metabolic profiling (2012)
  • Author(s): 入野 康宏、平田 悠、竹内 由紀子、波多野 直哉、竹縄 忠臣
  • Organizer: 85回日本生化学大会
  • Place of Presentation: 福岡・マリンメッセ福岡
  • Year and Date: 2012-12-15
• [Presentation] The establishment of metabolic proteome analysis by stable isotope (2012)
  • Author(s): 波多野 直哉、入野 康宏、竹縄 忠臣
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡・マリンメッセ福岡
  • Year and Date: 2012-12-11
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/icms/icms/index.html