2013 Fiscal Year Final Research Report
Development of vector specific to diffuse-type gastric cancer cells for peritoneal metastasis control
| Project/Area Number |
23501322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Clinical oncology
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
SASAKI Hiroki 独立行政法人国立がん研究センター, 研究所, ユニット長 (60235265)
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| Co-Investigator(Kenkyū-buntansha) |
SAEKI Norihisa 独立行政法人国立がん研究センター, 研究所, 主任研究員 (80466200)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 遺伝子治療 / びまん型胃がん |
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| Research Abstract |
We investigated the promoter activity of the GSDMB gene, which was identified to be expressed preferentially in diffuse-type gastric cancers (GCs). This promoter was found to act weakly in human mesothelial cell line MeT-5A but strongly in 5 of 6 diffuse-type GC cell lines. We next constructed the GSDMB promoter-driven HSVtk-lentivirus vector. This vector showed no cytotoxicity in MeT-5A cells, whereas 70% of diffuse-type GC-derived 60As6 cells were killed by Ganciclovir (GCV) treatment. We then established a peritoneal-metastasis control mouse model using 60As6 cells. By GCV treatment only once a week for 30 days after i.p. of 106 60As6 cells, we evaluated in vivo effects of this vector. The survival rate of GCV-treated mice showed 67%, but that of control mice showed 33%.
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[Journal Article] MicroRNA-148a is downregulated in gastric cancer, targets MMP7, and indicates tumor invasiveness and poor prognosis2014
Author(s)
Sakamoto N, Naito Y, Oue N, Sentani K, Uraoka N, Zarni Oo H, Yanagihara K, Aoyagi K, Sasaki H, Yasui W
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Journal Title
Cancer Sci.
Volume: 105
Pages: 236-243
Peer Reviewed
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[Journal Article] Intraperitoneal delivery of a small interfering RNA targeting NEDD1 prolongs the survival of scirrhous gastric cancer model mice2013
Author(s)
Fujita T, Yanagihara K, Takeshita F, Aoyagi K, Takigahira M, Chiwaki F, Fukagawa T, Katai H, Ochiya T, Sakamoto H, Konno H, Yoshida T, Sasaki H
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Journal Title
Cancer Sci.
Volume: 104
Pages: 214-222
Peer Reviewed
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[Journal Article] Analysis for thecombination expression of CK20, FABP1, and MUC2 is sensitive for the prediction of peritoneal recurrence in gastric cancer2012
Author(s)
Satoh Y, Mori K, Kitano K, Kitayama J, Yokota H, Sasaki H, Uozaki H, Fukayama M, Seto M, Nagawa H, Yatomi Y, Takai D
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Journal Title
Jpn. J. Clin. Oncol.
Volume: 42
Pages: 148-152
Peer Reviewed
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[Journal Article] FGFR2 gene amplification and clinicopathological features in gastric cancer2012
Author(s)
Matsumoto K, Arao T, Hamaguchi T, Shimada Y, Kato K, Ichiro Oda I, Taniguchi H, Koizumi F, Yanagihara K, Sasaki H, Nishio K, Yamada Y
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Journal Title
Br. J. Cancer
Volume: 106
Pages: 727-732
Peer Reviewed
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[Journal Article] Sensation of abdominal pain induced by peritoneal carcinomatosis is accompanied by changes in the expression of substance P and m-opioid receptors in the spinal cord of mice2012
Author(s)
Suzuki M, Narita M, Hasegawa M, Furuta S, Kawamata T, Miyano K, Yanagihara K, Chiwaki F, Ochiya T, Suzuki T, Matoba M, Sasaki H, Uezono Y
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Journal Title
Anesthesiology
Volume: 117
Pages: 847-856
Peer Reviewed
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[Journal Article] Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer2012
Author(s)
Sakamoto N, Oue N, Sentani K, Anami K, Uraoka N, Naito Y, Oo HZ, Ohdan H, Yanagihara K, Aoyagi K, SasakiH, Yasui W
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Journal Title
Cancer Sci.
Volume: 103
Pages: 1744-1750
Peer Reviewed
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[Journal Article] Chromosome 1q22 is a susceptibility locus of diffuse-type gastric cancer, harboring a functional SNP of MUC12011
Author(s)
Saeki N, Saito A, Choi JI, Matsuno K, Ohnami S, Totsuka H, Chiku S, Kuchiba A, Lee Y-S, Yoon K-A, Kook M-C, Park SR, Kim Y-W, Tanaka H, Tajima K, Hirose H, Tanioka F, Matsuno Y, Sugimura H, Kato S, Nakamura T, Nishina T, Yasui W, Aoyagi K, Sasaki H, Yanagihara K, Katai H, Shimoda T, Yoshida T, Nakamura Y, Hirohashi S, Sakamoto H
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Journal Title
Gastroenterology
Volume: 140
Pages: 892-902
Peer Reviewed
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[Journal Article] Artificially induced epithelial-mesenchymal transition in surgicasl subjects : its implications in clinical and basic cancer research2011
Author(s)
Aoyagi K, Minashi K, Igaki H, Tachimori Y, Nishimura T, Hokamura N, Ashida A, Daiko H, Ochiai A, Muto M, Ohtsu A, Yoshida T, Sasaki H
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Journal Title
PLoS One
Volume: 6(4)
Pages: e18196
Peer Reviewed
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[Journal Article] Signal peptidase complex 18, encoded by SEC11A, contributes to progression via TGF-a secretion in gastric cancer
Author(s)
Oue N, Naito Y, Hayashi T, Takigahira M, Kawano-Nagatsuma A, Sentani K, Sakamoto N, Zarni Oo H, Uraoka N, Yanagihara K, Ochiai A, Sasaki H, Yasi W
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Journal Title
Oncogene
Volume: (in press)
DOI
Peer Reviewed
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[Presentation] MiR-148a, down-regulated in gastric cancer, targets MMP7 and participates in tumor invasion and poor prognosis2013
Author(s)
Naoya Sakamoto, Yutaka Naito, Naohide Oue, Kazuhiro Sentani, Naohiro Uraoka, Htoo Zarni Oo, Kazuyoshi Yanagihara, Kazuhiko Aoyagi, Hiroki Sasaki, Wataru Yasui
Organizer
The 11^<th> ICGC
Year and Date
20130000
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[Presentation] Transforming growth factor-b enhances the effect of docetaxel via inducing asymmetric cell division of CXCR4^+ gastric cancer stem cells in a dormant state2013
Author(s)
Takeshi Fujita, Fumiko Chiwaki, Ryo-u Takahashi, Kazuyoshi Yanagihara, Kazuhiko Aoyagi, Hiromi Sakamoto, Takeo Fukagawa, Hitoshi Katai, Takahiro Ochiya, Manabu Ohta, Kinji Kamiya, Hiroyuki Konno, Teruhiko Yoshida, Hiroki Sasaki
Organizer
The 4^<th> JCA-AACR Special Joint Conference
Year and Date
20130000
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[Presentation] Multi-marker based RT-PCR for peritoneal cytology of gastric cancverMulti-marker based RT-PCR for peritoneal cytology of gastric cancver2011
Author(s)
Mori K, Sato Y, Kitayama J, Kaizaki S, Ishigami H, Yamaguchi H, Shimizu N, Nomura S, Wada I, Yokota H, Kageyama Y, Sasaki H, Uozaki M, Fukayama M, Yatomi Y, Takai D, Seto M
Organizer
The 9th IGCC(Kerea)
Year and Date
20110000
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