DNA damage induced by near ultraviolet (UVA) and signal transduction

2014 Fiscal Year Final Research Report

• Project/Area Number: 23510063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Okayama University
• Principal Investigator: NEGISHI Tomoe 岡山大学, 医歯(薬)学総合研究科, 准教授 (80116491)
• Co-Investigator(Kenkyū-buntansha): IBUKI Yuko 静岡県立大学, 食品栄養科学部, 教授 (30236781)
• Research Collaborator: FANG Xing ; TOYOOKA Tatsushi
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 近紫外光 / 紫外線 / DNA傷害 / 活性酸素種 / ヒストンリン酸化 / ショウジョウバエ / DNA傷害修復

Outline of Final Research Achievements

We have investigated the involvement of UVA in mutations and DNA damage in somatic cells using Drosophila melanogaster larvae. 365-nm-LED (a high-dose-rate UVA source) was mutagenic in mei-41 than in mei-9. The mei-41 gene was shown to be an orthologue of the human ATR gene, which is involved in the repair of DSBs through phosphorylation of histone H2Ax. LED-UVA was mutagenic in the urate-null strain at doses that were non-mutagenic in the urate-positive strain. We also observed that reactive oxygen species were produced in larvae by LED-UVA irradiation. At higher doses of LED-UVA, levels of phosphorylated H2AvD (γ-H2AvD) increased significantly in the urate-null strain but not in wild-type. It was reported that ATR functions on DSB repair in D. melanogaster. Taken together, we propose a possible pathway for UVA-induced mutation, whereby DNA double-strand breaks resulting from oxidative stress might be responsible for UVA-induced mutation in somatic cells of D. melanogaster larvae.

Free Research Field

生物化学