2013 Fiscal Year Final Research Report
Studies on genes relating to the formation of the testis-ovum in the testis of amphibians by the non-estrogenic action of environmental chemicals
| Project/Area Number |
23510077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Hiroshima University |
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Principal Investigator |
TAKASE Minoru 広島大学, 理学(系)研究科(研究院), 准教授 (80226779)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 精巣卵 / 両生類 / 性転換 / 性分化 / 生殖腺 / ビスフェノールＡ / ノニルフェノール |
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| Research Abstract |
In the present study, the formation of the testis-ovum has been investigated in amphibians exposed to environmental chemicals, such as bisphenol A (BPA), nonylphenol (NP), and estradiol benzoate (EB). Firstly, exposure experiments were performed using three species of amphibians. Abnormal testes were observed more remarkably in the tadpoles and metamorphosed froglets of the frog Rana rugosa than in those of the other species. Thus, R. rugosa was used as a model of an experimental animal in further experiments. Next, the testes were analyzed histologically in the tadpoles exposed to environmental chemicals to know the process of testis-ovum formation. Finally, genes expressed differentially in the tadpoles exposed to BPA were analyzed during the formation of the testis-ova. Genes encoding vitellogenin A1 and ERRgamma were isolated as candidates relating to the formation of testis-ova induced by the non-estrogenic action of the environmental chemical.
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Research Products
(9 results)
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[Journal Article] Establishment of transactivation assay systems using fish, amphibian, reptilian and human thyroid hormone receptors2013
Author(s)
Oka, T., Mitsui-Watanabe, N., Tatarazako, N., Onishi, Y., Katsu, Y., Miyagawa, S., Ogino, Y., Yatsu, R., Kohno, S., Takase, M., Kawashima, Y., Ohta, Y., Aoki, Y., Guillette, L. J. Jr., Iguchi, T
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Journal Title
J. Appl. Toxicol
Volume: 33
Pages: 991-1000
Peer Reviewed
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