toxicolgical role of Zn2+ in cell death induced by chemical stress

2013 Fiscal Year Final Research Report

• Project/Area Number: 23510078
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: The University of Tokushima
• Principal Investigator: OYAMA Yasuo 徳島大学, 大学院ソシオ・アーツ・アンド・サイエンス研究部, 教授 (80214229)
• Project Period (FY): 2011 – 2013
• Keywords: 細胞毒性 / 亜鉛イオン

Research Abstract

The toxicological role of Zn2+ in cell death induced by chemical compounds was examined in this study. Micromolar concentrations of Zn2+ potentiated the cytotoxicity of hydrogen peroxide while the chelator of intracellular Zn2+ diminished it. This result suggests an involvement of Zn2+ in the cell death induced by hydrogen peroxide. The chemical compounds that induced the increase in intracellular Zn2+ concentration potentiated the cytotoxicity of hydrogen peroxide. However, the increase in intracellular Zn2+ concentration by the chemical compounds elevated the cellular content of nonprotein thiols, cytoprotective substances. It is suggested that Zn2+ exerts reciprocal actions, cytoprotective and cytotoxic, on the cell death under certain conditions.

Research Products

• [Journal Article] Effects of tetracaine and lidocaine on intracellular Zn^<2+> levels in rat neurons : Preliminary analysis with FluoZin-3 fluorescence (2014)
  • Author(s): Eri Fukunaga, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 28, No. 1 Pages: 1-5
  • URL: http://web.ias.tokushima-u.ac.jp/bulletin/nat/nat28-1-1.pdf
  • Peer Reviewed
• [Journal Article] Triclocarban-induced change in intracellular Ca^<2+> level in rat thymocytes : Cytometric analysis with Fluo-3 under Zn^<2+>-free conditions (2014)
  • Author(s): Yukari Miura, Xiaohui Chen, Saki Yamada, Aya Sugihara, Molomjamts Enkhjargal, Yuanzhi Sun, Keiko Kuroda, Masaya Satoh, Yasuo Oyama
  • Journal Title: Environmental Toxicology and Pharmacology Volume: Vol. 37, No. 2 Pages: 563-570
  • DOI: 10.1016/j.etap.2014.01.013
  • Peer Reviewed
• [Journal Article] Clioquinol, a lipophilic Zn^<2+> chelator, augments and attenuates the cytotoxicity of H_2O_2: A bell-shaped response curve of the effects of the drug (2014)
  • Author(s): Tomohiro Oyama, Keisuke Oyama, Eri Fukunaga, Hitoshi Ishibashi, Yasuo Oyama
  • Journal Title: Toxicology Research Volume: Vol. 3, No. 2 Pages: 110-117
  • DOI: 10.1039/C3TX50097G
  • Peer Reviewed
• [Journal Article] Effect of triclocarban on membrane potential of rat thymocytes : Assessment with bis-(1,3-dibutylbarbituric acid) trimethine oxonol (2013)
  • Author(s): Yuanzhi Sun, Molomjamts Enkhjargal, Aya Sugihara, Saki Yamada, Xiaohui Chen, Yukari Miura, Eri Fukunaga, Masaya Satoh, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 27, No. 4 Pages: 51-57
  • URL: http://www.lib.tokushima-u.ac.jp/repository/metadata/105960
  • Peer Reviewed
• [Journal Article] Synergistic increase in cell lethality by dieldrin and H_2O_2 in rat thymocytes: Effect of dieldrin on the cells exposed to oxidative stress (2013)
  • Author(s): Tsolmon Chimeddorj, Tomoko Suzuki, Kazuhiro Murakane, Miyuki Inai, Masaya Satoh, Yasuo Oyama
  • Journal Title: Chemosphere Volume: Vol. 93, No. 2 Pages: 353-358
  • DOI: 10.1016/j.chemosphere.2013.04.092
  • Peer Reviewed
• [Journal Article] Role of Zn^<2+> in restoration of nonprotein thiol content in the cells under chemical stress induced by triclocarban (2013)
  • Author(s): Eri Fukunaga, Yasuhiro Kanbara, Yumi Hirao, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 27, No. 1 Pages: 1-5
  • URL: http://www.lib.tokushima-u.ac.jp/repository/metadata/104978
  • Peer Reviewed
• [Journal Article] Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress (2013)
  • Author(s): Yasuhiro Kanbara, Kazuhiro Murakane, Yumiko Nishimura, Masaya Satoh, Yasuo Oyama
  • Journal Title: The Journal of Toxicological Sciences Volume: Vol. 38, No. 1 Pages: 49-55
  • URL: https://www.jstage.jst.go.jp/article/jts/38/1/38.../_pdf
  • Peer Reviewed
• [Journal Article] Elevation of intracellular Zn^<2+> level by nanomolar concentrations of triclocarban in rat thymocytes (2012)
  • Author(s): Junpei Morita, Aoi Teramachi, Yosuke Sanagawa, Saramaiti Toyson, Hiroshi Yamamoto, Yasuo Oyama
  • Journal Title: Toxicology Letters Volume: Vol. 215, No. 3 Pages: 208-213
  • DOI: 10.1016/j.toxlet.2012.10.012
  • Peer Reviewed
• [Journal Article] Yttrium decreases the intracellular Zn^<2+> concentration in rat thymocytes by attenuating a temperature-sensitive Zn^<2+> influx (2012)
  • Author(s): Yusuke Takahashi, Kaori Kanemaru, Shoji Imai, Norikazu Miyoshi, Takuya Kawanai, Yasuo Oyama
  • Journal Title: Environmental Toxicology and Pharmacology Volume: Vol. 34, No. 2 Pages: 574-578
  • DOI: 10.1016/j.etap.2012.07.002
  • Peer Reviewed
• [Journal Article] A comparison of the cellular actions of polaprezinc (zinc-L-carnosine) and ZnCl_2 (2012)
  • Author(s): Hakaru Seo, Ikuko Ogata-Ikeda, Shiro Ishida, Yoshiro Okano, Yasuo Oyama
  • Journal Title: Life Sciences Volume: Vol. 90, No. 25-26 Pages: 1015-1019
  • DOI: 10.1016/j.lfs.2012.05.017
  • Peer Reviewed
• [Journal Article] Clioquinol-induced increase and decrease in the intracellular Zn^<2+> level in rat hymocytes (2012)
  • Author(s): Tomohiro Oyama, Shiro Ishida, Yoshiro Okano, Hakaru Seo, Yasuo Oyama
  • Journal Title: Life Sciences Volume: Vol. 91, No. 23-24 Pages: 1216-1220
  • DOI: 10.1016/j.lfs.2012.09.014
  • Peer Reviewed
• [Journal Article] Decrease in intracellular Zn^<2+> level by propranolol : A model experiment using rat thymocytes (2012)
  • Author(s): Yasuhiro Kanbara, Hakaru Seo, Yumiko Nishimura, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 26, No. 3 Pages: 11-16
  • URL: http://www.lib.tokushima-u.ac.jp/repository/file/96890/20120620154747/LID201206201003.pdf
  • Peer Reviewed
• [Journal Article] Nanomolar concentrations of zinc pyrithione increase cell susceptibility to oxidative stress induced by hydrogen peroxide in rat thymocytes (2012)
  • Author(s): Tomohiro Oyama, Minoru Saito, Takayasu Yonezawa, Okano Yoshiro, Yasuo Oyama
  • Journal Title: Chemosphere Volume: Vol. 87, No. 11 Pages: 1316-1322
  • DOI: 10.1016/j.chemosphere.2012.01.052
  • Peer Reviewed
• [Journal Article] Further analysis on lidocaine-induced increase in intracellular Zn^<2+> concentration : Cytometric model study using FluoZin-3, 5-chloromethylfluorescein, and rat thymocytes (2012)
  • Author(s): Yumiko Nishimura, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 26, No. 2 Pages: 7-10
  • URL: http://www.lib.tokushima-u.ac.jp/repository/file/96861/20120517114206/LID201205171003.pdf
  • Peer Reviewed
• [Journal Article] Triclosan, an antibacterial agent, increases intracellular Zn^<2+> concentration in rat thymocytes : Its relation to oxidative stress (2012)
  • Author(s): Ikumi Tamura, Yasuhiro Kanbara, Minoru Saito, Kanna Horimono, Masaya Satoh, Hiroshi Yamamoto, Yasuo Oyama
  • Journal Title: Chemosphere Volume: Vol. 86, No. 1 Pages: 70-75
  • DOI: 10.1016/j.chemosphere.2011.09.009
  • Peer Reviewed
• [Journal Article] Elevation of intracellular Ca^<2+> level by triclosan in rat thymic lymphocytes : Increase in membrane Ca^<2+> permeability and induction of intracellular Ca^<2+> release (2011)
  • Author(s): Ikumi Tamura, Minoru Saito, Yumiko Nishimura, Masaya Satoh, Hiroshi Yamamoto and Yasuo Oyama
  • Journal Title: Journal of Health Science Volume: Vol. 57, No. 6 Pages: 540-546
  • URL: http://jhs.pharm.or.jp/data/57(6)/57_540.pdf
  • Peer Reviewed
• [Journal Article] Some characteristics of membrane Cd^<2+> transport in rat thymocytes: An analysis using Fluo-3 (2011)
  • Author(s): Takuya Kawanai, Masahiro Fujinaga, Kazuki Koizumi, Isao Kurotani, Erika Hashimoto, Masaya Satoh, Shoji Imai, Norikazu Miyoshi, Yasuo Oyama
  • Journal Title: Biometals Volume: Vol. 24, No. 5 Pages: .903-914
  • DOI: 10.1007/s10534-011-9444-3
  • Peer Reviewed
• [Journal Article] Zinc-pyrithione exerts diverse actions on human leukemia K562 cells : A preliminary study on hormetic action (2011)
  • Author(s): Yosuke Endo, Zhehao Han, Jun Kurochi, Masahiro Sakata, Akinori Maetaka, Takayasu Yonezawa, Kazuki Koizumi, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 25, No. 3 Pages: 15-19
  • URL: http://web.ias.tokushima-u.ac.jp/bulletin/nat/nat25-3-1.pdf
  • Peer Reviewed
• [Journal Article] Putative role of intracellular Zn^<2+> release during oxidative stress : A trigger to restore cellular thiol content that is decreased by oxidative stress (2011)
  • Author(s): Akio Kinazaki, Hongqin Chen, Kazuki Koizumi, Takuya Kawanai, Tomohiro Oyama, Masaya Satoh, Shiro Ishida, Yoshiro Okano, Yasuo Oyama
  • Journal Title: The Journal of Physiological Sciences Volume: Vol. 61, No. 5 Pages: 403-409
  • DOI: 10.1007/s12576-011-0160-0
  • Peer Reviewed
• [Journal Article] Tetracaine decreases intracellular Zn^<2+> concentration by inhibiting Zn^<2+> influx in rat thymocytes (2011)
  • Author(s): Kaori Kimura, Yumiko Nishimura, Keisuke Oyama, Takuya Kawanai, Erika Hashimoto, Yasuo Oyama
  • Journal Title: Natural Science Research Volume: Vol. 25, No. 2 Pages: 7-13
  • URL: http://web.ias.tokushima-u.ac.jp/bulletin/nat/nat25-2-1.pdf
  • Peer Reviewed
• [Journal Article] NOR-3, a donor of nitric oxide, increases intracellular Zn^<2+> concentration and decreases cellular thiol content : A model experiment using rat thymocytes, FluoZin-3, and 5-chloromethylfluorescein (2011)
  • Author(s): Soya Harada, Chiharu Kubota, Natsuho Kitano, Takuya Kawanai, Kazuki Koizumi, Yasuo Oyama, Akio Kinazaki, Shiro Ishida and Yoshiro Okano
  • Journal Title: Natural Science Research Volume: Vol. 25, No. 1 Pages: 1-6
  • URL: http://web.ias.tokushima-u.ac.jp/bulletin/nat/nat25-1-1.pdf
  • Peer Reviewed