2013 Fiscal Year Final Research Report
Oriented enzyme immobilization on the electrode for biodevice
| Project/Area Number |
23510127
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | University of Fukui |
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Principal Investigator |
SUYE Shin-ichiro 福井大学, 工学(系)研究科(研究院), 教授 (90206376)
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| Co-Investigator(Kenkyū-buntansha) |
SATOMURA Takenori 福井大学, 大学院工学研究科, 准教授 (50412317)
SAKAMOTO Hiroaki 福井大学, テニュアトラック本部, 講師 (70552454)
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| Research Collaborator |
ZHENG Haitao 中国 天津工業大学, 材料化工学院, 副教授
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオデバイス / 分子配向 / 配向制御 / バイオ電池 / ナノデバイス |
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| Research Abstract |
The biodevice which could achieve an efficient enzyme reaction and electron transfer on the electrode surface was improved using the molecular orientated immobilization of enzyme. Both high-stable PQQ-dependent glucose dehydrogenase (PQQ-GDH) for bioanode and multicopper oxidase for biocathode from hyperthermophilic archaeon. In order to immobilize enzyme onto the electrode with orientation, His-tag was introduced to C-terminal of PQQ-GDH by genetic technique. Copper molecules were deposited on the electrode electrochemically, and then the enzyme was immobilized on the electrode by its affinity between His-tag and Cu atoms. The biofuel cells were constructed with both electrodes and the biofuel cell output a maximum power density of 15 microA/cm2. The results in the present study represent a potentially promising finding for the application to practical biofuel cells.
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Research Products
(16 results)
