Development of a potable ELISA machine using novel antigen-bound polymer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23510146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: University of Hyogo
• Principal Investigator: TAKEO MASAHIRO 兵庫県立大学, 工学(系)研究科(研究院), 准教授 (40236443)
• Co-Investigator(Kenkyū-buntansha): UTSUMI Yuichi 兵庫県立大学, 高度産業科学技術研究所, 教授 (80326298) ; YUSA Shinichi 兵庫県立大学, 大学院工学研究科, 准教授 (00301432)
• Co-Investigator(Renkei-kenkyūsha): NEGORO Seiji 兵庫県立大学, 大学院工学研究科, 教授 (90156159) ; KATO Dai-ichiro 兵庫県立大学, 大学院工学研究科, 助教 (60423901)
• Project Period (FY): 2011 – 2013
• Keywords: ELISA / nonylphenol / 分析機 / 内分泌撹乱物質

Research Abstract

We developed a portable ELISA machine for the measurement of an endocrine disruptor, nonylphenol. A microfilter, which was made from PMMA-based polymer with nonylphenyl group as a side chain, was set in a vertically-stacked microreactor. The microreactor was set in a newly-developed automatic fluidic and detection system in a 25cm-cubic case and competitive ELISA was performed using this small system. As a result, we could construct a good standard curve with negative correlation in a concentration range of 0.01-1.00 microgram/l and the average coefficient of variation of the data obtained was 4.2%. These results were comparable to the sensitivity and repeatability of commercial sensitive analytical machines.

Research Products

• [Presentation] Development of a portable fluidic and detection system for ELISA in a 3-dimentional microreactor (2013)
  • Author(s): M. Takeo, S. Nii, D.Kato, S. Negoro, S. Yusa, T. Saiki, Y. Takizawa, and Y. Utsumi
  • Organizer: Proc. of 39^<th> International Conference on Micro and Nano Engineering (MNE2013)
  • Place of Presentation: London, England
  • Year and Date: 20130916-19
• [Presentation] Development of automatic ELISA analytical system for a trace amount of nonylphenol7using a novel antigen-bound microfilter (2013)
  • Author(s): M. Takeo, I. Kawaji, A. Nakasuji, T. Tone, Y. Ukita, D. Kato, S. Negoro, S. Yusa, M. Katayama, and Y. Utsumi
  • Organizer: Proc. of 10^<th> International Workshop on High Aspect Ratio Micro and Nano Structure Technology (HARMNST2013)
  • Place of Presentation: Berlin, Germany
  • Year and Date: 20130421-24
• [Presentation] Automatic ELISA analytical system for a trace amount of environmental chemicals using a 3-dimensional microreactor with a novel antigen-bound microfilter (2012)
  • Author(s): M. Takeo, I. Kawaji, A. Nakasuji, T. Tone, Y.Ukita, D. Kato, S. Negoro, S. Yusa, M. Katayama, and Y.Utsumi
  • Organizer: Proc. of the 16^<th> International Conference on Miniaturized Systems for Chemistry and Life Science (μTAS2012)
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20121028-1001
• [Presentation] Three-dimentional Lab-on-CD with enzyme-linked immunosorbent assay (2011)
  • Author(s): T. Azeta, Y. Ukita, M. Takeo, N. Nakagawa, S. Yusa, and Y. Utsumi
  • Organizer: Proc. of 9^<th> International Workshop on High Aspect Ratio Micro Structure Technology (HARMST2011)
  • Place of Presentation: HsinChu, Taiwan
  • Year and Date: 20110612-18