2013 Fiscal Year Final Research Report
Analyses of genomic structure of gene amplification regions in human cancer cells and disease causation
| Project/Area Number |
23510241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATOH HITOSHI 東京大学, 新領域創成科学研究科, 准教授 (70183829)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 染色体 / FISH / 遺伝子増幅 / 癌 / ゲノム構造 |
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| Outline of Final Research Achievements |
In general, it happened apparently that a variety of gene amplification and deletion may interfere its expression and regulation. In the present study, I applied FISH technique to detect how the NOTCH3 gene is amplified in a mammary gland ductal carcinoma cell line of HC1143 and to make it narrow down a possible amplified genomic region commonly found in two of cell lines derived from primary effusion lymphomas. As a result, amplification of NOTCH3 gene was certified in HC1143 cells and a possible amplified region was narrowed down within the 1q25 region in PEL cell lines. However, disease causing candidate genes are not yet identified.
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| Free Research Field |
ゲノム科学
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